摘要
目的 探讨CD2 8协同结核杆菌 (M .tb)低分子多肽激活人外周血γδ+ T细胞时 ,其胞内信号的传递经过磷酸肌醇 3激酶 (PI3K)的介导作用。方法 用激发型抗CD2 8单抗模拟第二信号 ,与M .tb抗原一起刺激纯化的T细胞 ,同时用特异性PI3K抑制剂LY2 940 0 2阻断PI3K的活性 ,用流式细胞仪分析γδ+ T细胞上CD6 9分子的表达及γδ+ T细胞的增殖效应。结果 随着LY2 940 0 2浓度的增加 ,γδ+ T细胞表面CD6 9分子的表达率降低 ,其增殖效应亦被不同程度地阻断。结论 抗CD2 8单抗可协同M .tb抗原激活γδ+ T细胞 ,其活化信号在γδ+ T细胞内的转导经过PI3K的介导。
Objective To explore the role of phosphatidylinositol 3 kinase (PI3K) in the intracellular signaling transduction pathways of human peripheral blood γδT cells activated by CD28 with Mycobacterium tuberculosis (M.tb) low molecular peptide antigen. Methods Flow cytometry was used to detect the expressions of CD69 molecule on activated γδT cells and proliferation rates of γδT cells stimulated by anti-CD28 monoclonal antibody and M.tb antigen. Meanwhile, the PI3K inhibitor LY294002 was added to block the activity of PI3K to find the effect on expressions of CD69 molecule and γδT cells proliferation response. Results The expression of CD69 molecule on γδT cells and proliferation response of γδT cells were blocked by addition of LY294002. Conclusion The intracellular signaling pathways delivered by CD28 and M.tb antigen in γδT cells was transducted by PI3K. [
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2003年第2期123-126,共4页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金资助项目 ( 30 0 70 72 1)
安徽省自然科学基金资助项目 ( 990 445 42 )
