摘要
目的 :对目前免疫组织化学染色中应用最为广泛的Envision二步法与LSAB三步法进行比较。方法 :将本科常规肿瘤活检标本固定 ,脱水 ,石蜡包埋 ,切片 ,分别应用CK、Envision法和LSAB法进行相应的 30多种不同单克隆 /多克隆抗体免疫组化染色。结果 :两种方法特异性强 ,阳性定位准确 ,无差异。Envision法敏感度及阳性强度均优于LSAB法 ,操作更为简便 ,更省时 ,背景清晰 ,非特异性染色少 ,缺点是试剂盒在价格上略高于LSAB法。结论 :Envision二步法特别适合于快速临床病理诊断 。
Objective:To compare Envisions and LSABs methods that are currently applied widely in immunohistochemistly staining. Method: To fixate,dehydrate,wax and section the usual samples from tumor biopsy.To make the corresponding thirty or more species of monoclonal/polyclonal antibody immunohistochemisty staining respectively with Envisions and LSABs methods. Result: The two methods have no difference in high specificity and exact positive location. The sensitivity and positive intensity of Envisions method are better than that of LSABs. The former is operated more briefly and faster,with clear setting and little non specific staining,but is more expensive than LSABs method for the test kit. Conclusion: Envisions method is especially favorable in fast clinical pathologic diagnosis,while LSABs method is more effective for scientific research or ratio trial.
出处
《广州医学院学报》
2002年第4期50-52,共3页
Academic Journal of Guangzhou Medical College
