摘要
目的:探讨荧光原位杂交(FISH)技术检测慢性淋巴细胞白血病(CLL)患者染色体异常的临床价值。方法:运用FISH技术,采用5种特异性DNA探针检测46例CLL患者的染色体异常,并结合患者的临床资料,分析各种染色体异常在临床诊断及预后判断中的价值。结果:46例CLL患者中,32例出现染色体异常,总异常检出率为69.6%,其中del(11q22.3)8例(17.4%),del(13q14.3)14例(30.4%),P53缺失7例(15.2%),del(13q14)5例(10.9%),CSP12阳性10例(21.7%)。结论:FISH技术检测染色体异常的敏感性和特异性较高。del(13q14)是CLL患者最常见的染色体异常。存在基因异常的患者预后较正常者差,尤其是存在P53基因缺失的患者。
Objective:To investigate the clinical significance of the detection of chromosomal abnormalities in chronic lymphocytic leukemia(CLL)patients by fluorescence in situ hybridization(FISH)technology.Method:Using five kinds of specific DNA probes to detect the chromosomal abnormalities in 46CLL patients and combined with clinical data,we analyzed the value of each chromosomal abnormality in the clinical diagnosis and prognosis.Result:Thirty-two patients showed chromosomal abnormalities,with total abnormal rates 69.6%.The positive rates of del(11q22.3),del(13q14.3),P53deletion,del(13q14),trisomy 12were 17.4%,30.4%,15.2%,10.9%and 21.7%,respectively.Conclusion:The sensitivity and specificity of FISH technique for the detection of chromosomal abnormalities are both high.del(13q14)is the most common chromosome abnormality in CLL patients.The prognosis of patients with abnormal FISH results is significantly worse than that of normal ones.P53gene can affect the prognosis of CLL patients mostly.
出处
《临床血液学杂志》
CAS
2014年第A02期240-243,共4页
Journal of Clinical Hematology
基金
卫生部科研课题(No:WKJ 2007-3-001)
关键词
慢性淋巴细胞白血病
荧光原位杂交
细胞遗传学异常
chronic lymphocytic leukemia
fluorescence in situ hybridization
cytogenetic aberrations
