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组织工程方法构建复合培养细胞的引导组织再生膜 被引量:8

Reconstruction of the guided tissue regeneration membrane combined with cultured cells by tissue engineering
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摘要 目的 :探讨可吸收引导组织再生胶原膜 (BME10 -X)的生物相容性 ,构建引导组织再生膜。方法 :用组织工程方法将人牙周膜成纤维细胞与BME10 -X复合培养 ,培养第 7、14d进行组织学及扫描电镜、透射电镜观察。结果 :人牙周膜成纤维细胞可在BME10 -X上贴附、增殖 ,分泌大量的细胞外基质 ,复层生长形成纤维组织结构 ,基本形成具有活性细胞及其基质的膜样组织 ,BME10 -X在培养一周即出现可见的吸收。结论 :BME10 -X具有良好的生物相容性 。 AIM:To evaluate the guided tissue regeneration collagen membrane (BME10-X) as tissue engineering scaffolds for periodontium formation. METHODS:Human periodontal ligament fibroblasts(HPDLFs) were cultured combined with the BME10-X in vitro .Histologic examination was performed with light microscopy.scanning electron microsopy and transmission electron microscopy at 7 and 14 day.RESULTS:Seeded HPDLFs were well attached to BME-10X and proliferated in a multi-layer fashion.Membrane-like tissue was observed at 14 day.CONCLUSION: BME-10X has a good biocompatibility with HPDLFs, it can be used as scaffold for periodontal tissue engineering.
出处 《牙体牙髓牙周病学杂志》 CAS 2003年第3期139-141,共3页 Chinese Journal of Conservative Dentistry
基金 福建省科技厅重大课题资助 (2 0 0 1Z0 2 1)
关键词 牙周膜成纤维细胞 生物材料 生物相容性 组织工程 periodontal ligament fibroblasts biomaterials biocompatibility tissue engineering
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