摘要
目的:研究氨茶碱对临床常见变应原粉尘螨致敏诱发的哮喘小鼠气道炎症及树突状细胞(DC)密度、分布的影响。方法:粉尘螨腹腔注射与雾化吸入诱发BALB/c小鼠哮喘发作,末次吸入24 h后采用免疫组织化学及计算机图像分析方法比较各组小鼠气道DC的分布及密度改变,并测定支气管肺泡灌洗液(BALF)中细胞总数、嗜酸性粒细胞(EOS)计数及IL-5(ELISA测定)浓度。结果:粉尘螨诱发后哮喘小鼠BALF中EOS计数(3.29±1.38)×1 06/L、IL-5水平(25±5)pg/ml均较正常组EOS计数(0.05±0.01)×106/L、IL-5水平(6±3)pg/ml显著升高(P<0.05、P<0.01),气道DC密度个数每平方毫米上皮面积(1542±28)个与正常组小鼠每平方毫米上皮面积(600±20)个比较,差异有显著性(P<0.01)。小鼠经小剂量氨茶碱处理后气道DC密度、BALF中EOS计数和IL-5水平与哮喘组比较,差异有显著性(P<0.05)。各组小鼠肺内DC分布未见明显变化。结论:小剂量氨茶碱抑制EOS性气道炎症,下调气道DC密度,未影响DC肺内分布。
Objective: To investigate the effect of aminophylline on airway inflammation induced by dermatophagoides farinae and the distribution and density of airway intraepithelial dendritic cell (DC) in murine models. Methods: The animal model of asthma was parepared by sensitivity and exposition of dermatophagoides farinae, the pulmonary and tracheal samples and bronchoalveolar lavage (BAL) were examined at 24 h after last challenge. The method of immunohistochemistry was used to detect the alteration of distribution and density of DC, and transmission electron microscope and computer image analysis were applied to analyse the morphology of DC. The cell count, and the count of eosinophil(EOS) in bronchoalveolar lavage fluid(BALF) were examined. IL-5 in BALF was detected by ELISA. Results: The EOS count and the level of IL-5 in BALF of the asthmatic models, were more increased than that of the controls[(3. 29±1.38)×106/L vs (0.05±0.01)×106/L and (25±5)pg/ml vs (6±3)pg/ml, P < 0. 05, P < 0. 01]. There was significant difference on the density of airway DC between the asthmatic group and the controls[(1542±28)cells/mm2 vs (600±20) cells/mm2, P < 0. 01] . And there was also significant difference on the density of airway DC and EOS count and IL-5 in BALF between aminophylline-treatment group and asthmatic group ( P < 0. 05) . In three groups, there was no obviously alteration of the distribution of airway DC. Conclusion: Low concentration aminophylline can down-regulate the amounts of dendritic cell and inhibit airway inflammation in murine lung.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第2期151-153,F003,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
中华医学会迈特兴华茶碱基金资助项目 (KA0201)
