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载脂蛋白(a)基因TTTTA五核苷酸重复序列多态性的分析及与血清脂蛋白(a)水平的关系 被引量:2

The Relation of TTTTA Pentanucleotide Repeats Polymorphisms of Apolipoprotein(a) Gene and Serum Lipoprotein(a)
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摘要 目的:探讨南京地区汉族人群中载脂蛋白(a)基因TTTTA五核苷酸重复序列多态性及与血清脂蛋白(a)水平的关系。方法:采用PCR、变性聚丙烯酰胺凝胶电泳和溴乙锭染色等技术分析南京地区156例正常汉人TTTTA五核苷酸重复序列。结果:共检出TTTTA串联次数为5、7、8、9、10和11共6种等位基因和5/9、5/8、7/10、8/8、8/9、8/10、9/9、9/10、9/11、11/11的10种基因型,8等位基因频率在南京地区汉族人群中最高,9及5次之。5等位基因(5/9+5/8)血清脂蛋白(a)水平高于8(8/8)、9(8/9+9/9),但未见有统计学差别。结论:中国汉族南京地区人群存在载脂蛋白(a)基因TTTTA五核苷酸重复序列多态性;未能发现载脂蛋白(a)基因型血清脂蛋白(a)水平的差别。 Objective: To expolre the relation between TTTTA pentanucleotide repeats (PNR) polymorphisms of apolipoprotein(a) [apo(a)] gene and serum lipoprotein(a) in the people of Nanjing area. Methods: The techniques of polymerase chain reaction (PCR) denature polyacrylamide gel electrophoresis and ethidium bromide stain were used to determine the TTTTA PNR polymorphism of apo (a) gene in 156 healthy subjects of Chinese Han people in Nanjing. Results: The Tandem frequency of TTTTA PNR in Nanjingers of Han nationality has 6 alleles of 5、7、8、9、10、11 and 10 genotypes of 5/9、5/8、7/10、8/8、8/9、8/10、9/9、9/10、9/11 and 11/11; the frequency of 8 allele was remarkably higher than 9 and 5. The serum lipoprotein(a) in allele 5(5/9 +5/8) was higher than 8(8/ 8) and 9(8/9 +9/9), but there was no different in statistics. Conclusion:There was polymorphism of TTTTA PNR in people of Han nationality in Nanjing aera; there was no difference in serum lipoprotein(a) among the genotypes of TTTTA PNR polymorphism.
出处 《南京医科大学学报(自然科学版)》 CAS CSCD 北大核心 2003年第2期122-123,141,共3页 Journal of Nanjing Medical University(Natural Sciences)
基金 江苏省教委自然科学基金资助项目(015BC9804)
关键词 载脂蛋白 多态性 聚合酶链反应 血清 脂蛋白 心脑血管病 apolipoprotein(a) polymorphisms polymerase chain reaction
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  • 1Xue S, Green MA, LoGrsso PV, et al. Comparison of the effects of Apo(a) kringle Ⅵ-10 and plasminogen Kringles on the interactions of lipoprotein(a) with regulatory molecules[J]. Thromb Haemost, 1999, 81 (3): 428 - 435. 被引量:1
  • 2Verges BL. Dyslipidaemia in diabettes mellitus. Review of the main lipoprotein abnormalities and their consequences on the develpomentof atherogenesis[J]. Diab Metab, 1999, 25 (supp13): 32 - 40. 被引量:1
  • 3胡波,周新,邵华.载脂蛋白(a)五核苷酸串联重复序列基因型的检测[J].中华检验医学杂志,2000,23(2):83-86. 被引量:5
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