摘要
介绍了一种自制微透析探针的制备方法,考察了灌流液流速、温度以及探针膜面积等因素对金属离子Ni(Ⅱ)回收率的影响。实验结果表明:自制微透析探针透析金属离子的各种指标都与商品的微透析探针性能相当,可以替代商品化微透析探针研究金属离子与蛋白质的相互作用。利用微透析与高效液相色谱联用技术研究了Ni(Ⅱ)与人血清白蛋白(HSA)的相互作用。用自制的微透析探针测定Ni(Ⅱ)与HSA之间的第一结合位点数和结合常数分别为1 16和8 6×105L/mol,采用商品的微透析探针测定的结果分别为1 23和7 5×105L/mol。
A method for the preparation of the microdialysis probe is reported. Effects of the perfusion rate, the temperature and the area of the microdialysis probe membrane on the recovery of nickel ion were studied. The selfmade microdialysis probes showed characteristics similar to those of the commercial ones. The interaction of Ni(Ⅱ) with human serum albumin (HSA) was studied by these microdialysis probes coupled with HPLC. With the selfmade and commercial probes, the number of primary binding sites of Ni(Ⅱ) on HSA was found to be 116 and 123, respectively, and the binding constant was found to be 86×105 L/mol and 75×105 L/mol, respectively. The results suggest that the inexpensive selfmade microdialysis probes could be used as the substitutes of expensive commercial probes.
出处
《色谱》
CAS
CSCD
北大核心
2003年第2期147-150,共4页
Chinese Journal of Chromatography
基金
国家自然科学基金资助项目(批准号:29725512).
