摘要
为了分析脊椎动物从鳍到肢转变过程中基因的系统进化,采用PCR法克隆了斑马鱼和矛尾鱼的Hoxa-11基因片段,测序并进行序列分析.结果显示同源异型盒所在的外显子 区和剪接位点是高度保守的,外显子 区又可分为四个亚区:中度保守区、可变区和两个高度保守区.从鱼到四足动物,Hoxa-11基因的主要变化是可变区的长度梯增且出现富含丙氨酸的区域.此外,在内含子中也发现了两个高度保守的DNA序列,其长度分别为35bp和16bp.
In order to study the evolution of the vertebrate Hox genes during the fin limb transition, Hoxa11 genes were amplified by PCR, clo ned and sequenced from two fishes, zebrafish (Danio rerio) and latimeria (Latimeria chalumnae) . Sequen ce analysis showed that exon 2 including the homeobox and the splice site are hi ghly conserved in all aligned Hoxa11 genes. Exon 1 could be di vided into four r egions: two highly conserved regions, a moderately conserved region and a variab le region. Principal changes from fish to tetrapod, are th e gradual increase in length and the appearance of the rich in alanine subre gion in the variable region a c cording to the sequence alignment. In addition, 35bp and 16bp sequences, which are 100% conserved in all but zebrafish, were found in intron.
出处
《浙江大学学报（工学版）》
EI
CAS
CSCD
北大核心
2003年第2期239-242,共4页
Journal of Zhejiang University：Engineering Science
基金
王宽诚教育基金资助项目.
