摘要
目的 :研究p5 3、C -erbB - 2基因蛋白和细胞增殖核抗原 (PCNA)的表达与细支气管肺泡细胞癌 (bronchioloaveolarcarcinoma ,BAC)临床病理特征的关系。方法 :应用免疫组化法检测 36例BAC(局限型 2 5例 ,弥漫型 11例 )组织中 p5 3、C -erbB - 2、PCNA的表达水平。结果 :弥漫型 p5 3阳性率和PCNA增殖指数分别为 81.82 %和 72 .2 3± 8.5 9,明显高于局限型36 .0 0 %和 4 6 .14± 6 .2 0 ,(P <0 .0 5 )。C -erbB - 2阳性表达在两型中无明显差异 ,p5 3和PCNA阳性表达均与淋巴结转移有关 ,而且PCNA表达亦与P -TNM分期密切相关 (P <0 .0 5 )。弥漫型伴淋巴结转移的发生率和淋巴结阳性数目 (72 .73% ,平均 2 .6 3枚 )较局限型者 (5 2 .0 % ,平均 2 .0枚 )高 ,其 p5 3、C -erbB - 2阳性表达的程度亦较局限型有或无淋巴结转移者强。 结论 :p5 3基因蛋白和PCNA的表达对BAC预后的评价有一定的参考价值 。
Objective: To study the clinical significance and expression of p53,C-erbB-2 and PCNA in bronchioloaveolar carcinoma (BAC). Methods: Thirty-six cases of BAC were collected (25 cases localized type and 11 cases diffused type).The expression of p53,C-erbB-2 and PCNA in BAC tissues was determined with immunohistochemical methods. Results: The positive rate of p53 gene protein expression and PCNA proliferating index in the diffused type were 81.82% and 72.23±8.59 respectively,which were both significantly higher than 36.00% and 46.14±6.20 of those in the localized type ( P< 0.05);there was no significant difference on C-erbB-2 in the localized type and diffused type;the positive expression of p53 and PCNA was significantly related to the lymph node metastasis,and PCNA proliferating index was closely related to the P-TNM staging ( P <0.05).The lymph node numbers and positive rate (2.63,72.73%) of the diffused type with the lymph node metastasis were higher than those (2.0,52.0%) in the localized type and the positive expression of p53,C-erbB-2 gene protein were more severe than the localized type with or not. Conclusion: The expression of p53and PCNA could be quite valuable in the prognostic evaluation of BAC and the diffuse BAC could possess more biological characteristics of the malignant tumor.
出处
《重庆医科大学学报》
CAS
CSCD
2003年第2期205-207,212,共4页
Journal of Chongqing Medical University
