摘要
以1％蛋黄和5％蛋清代替血清制成培养基培养支原体和在培养基中加入溴甲酚紫作pH指示剂观察其生化反应。结果表明,支原体生长迅速且菌落形态典型,溴甲酚紫变色pH阈较低,能更确切地反映支原体生长和代谢活动。莱氏支原体能发酵葡萄糖和乳糖产酸,能还原TTC。但不发酵蕈糖和不吸附红细胞。
In order to simplify the mycoplasma culture medium preparation, an egg medium contained 1% yolk and 5% egg white was made. Bromoresol purple was substituted for phenol red as a pH indicator. On this medium, acholeplasma laidlawii grew well, formed typical colony, fermented glucose and lactose, but not trehalose dihydrate. It also reduced TTC, but not be absorbed to chick red blood cell.
出处
《贵阳医学院学报》
CAS
1992年第4期304-307,共4页
Journal of Guiyang Medical College
关键词
支原体属
培养基
卵白
蛋黄
mycoplsma
culture medium
egg white
egg yolk
