摘要
目的探讨p16表达对软骨肉瘤诊断,分级和预后的临床意义。方法应用免疫组化(S-P法)检测p16在软骨肉瘤石蜡切片中的表达,再用流式细胞仪检测软骨肉瘤DNA倍体。分析p16蛋白表达与良、恶性软骨肿瘤和软骨肉瘤病理分级间以及与软骨肉瘤不同临床病理因素间的关系。结果 (1)p16表达与软骨肉瘤组织学分级显著相关(P<0.05)。(3)p16表达在软骨肉瘤体积、复发差异有显著性(P<0.05)。结论p16蛋白表达对软骨肉瘤组织学分级,恶性进展和预后判断有指导意义。
Objective To investigate the clinical significance of p16 protein expression in the diagnosis,histological grading and prognosis of chondrosarcoma. Methods p16 expression was studied in paraffin sections of chondrosarcoma by immunohistochemical (S-P) assay,and DNA ploidy was measured by flow cytometry. Results (1) p16 protein expression showed significant correlation with his-tologic grades of chondrosarcoma (P<0.05). (2) Normal cartilaginous tissue,benign cartilaginous tumor, and low grade (grade Ⅰ and grade Ⅱ) chondrosarcoma were mainly diploid. High grade (grade Ⅲ) chondrosarcoma was aneuploid. The higher p16 protein expressed, the less DNA aneuploid presented. p16 expression showed a significant direct correlation with DNA aneuploid (r=0.925,P< 0.05). (3) There were statistical significances (P<0.05) in comparing the differences between p16 protein expression and tumor size and recurrence. Couclusion p16 protein expression is of some guiding significance in histologic grading,malignant development and prognostic assessment for chondrosarcoma.
出处
《肿瘤》
CAS
CSCD
北大核心
2003年第1期62-64,共3页
Tumor
