摘要
目的 探讨甘氨酸对急性坏死性胰腺炎并发的肺损伤的保护作用。方法 雄性Wistar大鼠随机分为假手术组、胰腺炎组、甘氨酸预处理组 (1g kg)和甘氨酸治疗组 (1g kg)。测定成模后 6,12h和 2 4h血清TNF α和肺匀浆中谷胱甘肽 (GSH )、髓过氧化物酶 (MPO)水平 ,观察肺组织学病变并比较各组动物 3d生存率。结果 甘氨酸预处理可明显降低各时相TNF α水平 ,甘氨酸治疗可降低 2 4h血清TNF α水平 ;甘氨酸预处理或治疗均不能提高各时相降低的肺GSH水平 ,但可降低 12h和 2 4h肺MPO活性 ,明显改善肺病变 ,提高生存率。结论 甘氨酸虽然不能提高肺GSH水平 ,但对急性坏死性胰腺炎并发的肺损伤仍有一定的防治作用 ,可能与抑制白细胞浸润激活有关。
Objective To investigate the protective effects of glycine on the lung injury in acute necrotizing pancreatitis(ANP). Methods Male Wistar rats were randomly divided into 4 groups: sham operation, ANP group,pretreated group and treatment group(with intravenous glycine 1g/kg). Serum TNF α level,glutathione (GSH) and myeloperoxidase (MPO) in pulmonary homogenate were determined at 6, 12, and 24h hours after ANP induced. Pulmonary pathology and 3 day survival rate were compared between the groups.Results Compared with ANP group,in glycine pretreated group serum TNF α level significantly reduced at 6,12, and 24h,while in glycine treatment group serum TNF α level decreased at 24-hour . In glycine pretreated group or treatment group, the pulmonary GSH content were decreased.In both glycine pretreated group and glycine treatment group, pulmonary MPO activity decreased at 12 and 24h,and pulmonary pathologic severity significantly ameliorated and survival rate increased. Conclusions Although glycine can not increase the GSH synthesis,but giving prophylactic or therapeutic glycine may be effective in improving acute lung injury in ANP, probably through the inhibition of the infiltration and activation of leukocytes.
出处
《中国普通外科杂志》
CAS
CSCD
2003年第2期116-118,共3页
China Journal of General Surgery
