摘要
目的了解临床分离的泛耐药鲍曼不动杆菌中氨基糖苷修饰酶(AME)及碳青霉烯酶编码基因存在状况。方法对临床分离的鲍曼不动杆菌(Ab)用K-B法检测16种常用抗菌药物的敏感性,用PCR检测OXA-23群、OXA-24群碳青霉烯酶和6种AME(aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6′)-Ⅰ、aac(6′)-Ⅱ、ant(3″)-Ⅰ、ant(2″)-Ⅰ)编码基因。结果79株Ab的药敏结果表明,59株菌(74.7%)属于多重耐药株(MDRA),42株菌(53.2%)属于泛耐药株(PDRA)。79株菌株中OXA-23群基因阳性33株(41.8%),从39株Ab中有29株检出AME基因阳性,包括aac(3)-Ⅰ阳性25株(64.1%)、aac(6′)-Ⅰ阳性25株(64.1%)、ant(3″)-Ⅰ阳性29株(74.4%),并且大多数菌株为多种基因阳性。AME阳性菌全部为OXA-23群基因阳性株,并且全部携带2～3种AME基因。结论本地临床分离的Ab耐药性非常严重,PDRA主要携带OXA-23群碳青霉烯酶基因,并且普遍携带AME编码基因。
Objective To investigate the genotyping of aminoglycoside modifying enzymes on pan-drug resistant A.baumannii(PDRA)isolated.Methods The antibiotic susceptibility of 16 different antibiotics of A.baumannii were tested by K-B method,aminoglycoside modifying enzymes coding genes of A.baumannii were detected by PCR.Results The detection rate of OXA-23group was 41.8%and the detection rates of aminoglycoside modifying enzymes coding genes of aac(3)-Ⅰ,aac(6’)-Ⅰand ant(3″)-Ⅰ were 64.1%,64.1% and 74.4%,respectively.Conclusion The study showed that it is more serious for PDRA carrying aminoglycoside modifying enzymes coding genes.
出处
《浙江检验医学》
2007年第4期28-30,共3页
Zhejiang Journal of Laboratory Medicine
关键词
泛耐药
鲍曼不动杆菌
氨基糖苷修饰酶
基因
Pan-drug resistant
Acinetobacter baumannii
Aminoglycoside modifying
Enzymes genes
