摘要
目的观察不同损伤程度的多发伤患者外周血免疫与炎症标志的关系,以探讨不同程度损伤中病理生理变化的意义。方法用流式细胞术对58例多发伤患者外周血T细胞亚群和单核细胞表面人类白细胞抗原DR(HLA-DR)的百分率进行检测,同时检测外周血白细胞数及C-反应蛋白水平。结果多发伤重度组外周血单核细胞表面HLA-DR的百分率低于轻度组(P【0.05)。在轻度组中,外周血CRP水平与CD8+/CD3+T淋巴细胞、单核细胞表面HLA-DR均呈明显的负相关(r=-0.569,P=0.003;r=-0.681,P【0.001);而在重度组中,外周血白细胞计数与单核细胞表面HLA-DR呈明显的负相关(r=0.854,P【0.001)。结论严重创伤患者免疫功能受到抑制,单核细胞表面HLA-DR的百分率与炎症因子CRP、WBC有相关性;炎症因子及免疫功能紊乱可能是影响多发伤患者病情进展的重要因素。
Objective To study on peripheral blood T-lymphocyte subpopulations and inflammation in patients with multiple trauma and to explore the process between them.Methods Patients with multiple trauma (n=58) were included in this study, peripheral blood T-lymphocyte subpopulations and the percentage of cells expressing human leukocyte antigen-DR(HLA-DR) on monocytes were determined by flow cytometric analysis. Scatter turbidimetry was used to measure the serum level of CRP.Results The HlA-DR expression on monocytes was significantly decreased in severe trauma group versus moderate trauma group (P<0.05) . There was negative correlation between the serum CRP peak level and the percentage of cells expressing HLA-DR and CD8+T-lymphccyte (r=-0.569, P=0.003, r=-0.681, P<0.001) in moderate trauma group. There was engative correlation between the WBC level and the percentage of cells expressing HLA-DR (r=-0.854, P<0.001) in severe trauma group.Conclusion Immune function is suppressed in patients with multiple trauma of different grade and the percentage of cells expressing HLA-DR on monocytes correlates with CRP, WBC. The inflammatory factors and the immunologic mechanism may both play important roles in development of multiple trauma.
出处
《浙江检验医学》
2007年第3期13-15,共3页
Zhejiang Journal of Laboratory Medicine
关键词
多发伤
免疫
炎症
单核细胞
Multiple trauma
Immune
Inflammation
Monocytes
