摘要
本试验用50日龄Wistar纯系雄性大鼠 ,采用放射性同位素示踪技术结合灌注体内原位结扎肠段技术 ,研究了饲粮中添加高剂量铜对氯化亚铁、赖氨酸螯合铁和甘氨酸螯合铁吸收的影响。试验中观察了向结扎肠段灌注含不同形态铁的吸收液后 ,不同时间点(5、15、30、60、90、120分钟)血液中59Fe比放射性的动态变化 ;120分钟(试验结束)时不同组织器官中59Fe的放射性大小和总铁含量以及主要血液学指标的变化。试验结果表明 :①在一定时间内(28天) ,采食缺铁纯合日粮和纯合高铜日粮(250mg/kg) ,大鼠体内铁的耗竭程度有显著差异 ,纯合高铜日粮使大鼠体内铁耗竭程度更大。②在本试验条件下 ,向日粮中添加高剂量铜(250mg/kg) ,对氯化亚铁、赖氨酸螯合铁和甘氨酸螯合铁的吸收影响都不显著。③与氯化亚铁相比 。
Using the technique of perfusing ligated intestinal segment,in vivo,in situ and radioactive isotope tracing,one study was conducted to determine iron absorption of iron amino acid chelate(Fe-Lys and Fe-Gly)with 50 days Wistar male rats.The radioactive concentrations of 59Fe in blood at 5,15,30,60,90 and 120 minutes after perfusing different tested solutions,and in different tissues and organs at 120 minutes were determined. After killing the rats,the iron concentrations in different tissues and organs and main parameters of haematalogy at 120 minutes were analyzed.The results indicated that:①The degree of depletion of iron in the rat fed low iron purified diet for 28 days was significantly lower than in the rat fed high copper purified diet.②Under the condition of this study,the effect of high dietary Cu on the iron absorption in FeCl2,Fe-Lys and Fe-Gly was not significant.③Compared with iron in FeCl2,the rats could effectively absorb iron in Fe-Lys and Fe-Gly.
出处
《动物营养学报》
CAS
CSCD
2003年第1期15-20,共6页
CHINESE JOURNAL OF ANIMAL NUTRITION
