摘要
目的探讨p62基因沉默对宫颈癌Hela细胞生长和对顺铂敏感性的影响。方法选取宫颈癌Hela细胞株,随机分为对照组、空白shRNA组和p62-shRNA组,其中空白shRNA组转染空白慢病毒载体,p62-shRNA组转染沉默p62表达的慢病毒载体,采用RT-PCR检测各组p62 mRNA表达,CCK法检测各组细胞增殖以及在不同顺铂浓度下细胞存活情况,采用流式细胞仪检测各组细胞凋亡情况。结果 p62-shRNA组p62 mRNA相对表达量为(0.241±0.096),明显低于对照组和空白shRNA组(P<0.05);p62-shRNA组培养24h、48h、72h细胞OD值明显低于对照组和空白shRNA组(P<0.05);p62-shRNA组细胞凋亡率为(23.20±2.91)%,明显高于对照组和空白shRNA组(P<0.05);0.05mg/L、0.5mg/L和5mg/L顺铂作用下,p62-shRNA组细胞存活率分别为(60.11±3.10)%、(34.38±2.91)%和(22.10±3.00)%,明显低于对照组和空白shRNA组(P<0.05)。结论 p62基因沉默可抑制宫颈癌Hela细胞增殖,促进细胞凋亡,增加对顺铂的敏感性。
Objective To investigate the effect of p62 gene silencing on the growth and cisplatin sensitivity of cervical cancer Hela cells.Methods Cervical cancer Hela cell lines were selected and randomly divided into control group,blank shRNA group and p63-shRNAgroup.The blank shRNA group was transfected with blank lentiviral vector and p62-shRNA group transfected with lentivirus vector which silenced p62 expression.The expression of p62 mRNA in each group was detected by RT-PCR.The cell proliferation of each group and the survival of cells in different concentrations of cisplatin were detected by MTT method.The apoptosis of each group was detected by flow cytometry.Results The relative expression of p62-mRNA group was(0.241±0.096),which was significantly lower than that in control group and blank shRNA group(P<0.05);The OD value of p62-shRNA group was significantly lower than those in control group and blank shRNA group at 24 h,48 h and 72 h(P<0.05);The apoptotic rate of p62-shRNA group was(23.20±2.91)%,which was significantly higher than those in control group and blank shRNA group(P<0.05);At concentrations of 0.05 mg/L,0.5 mg/L and 5 mg/L cisplatin,the cell viability of p62-shRNA group was(60.11±3.10)%,(34.38±2.91)%and(22.10±3.00)%,respectively,which was significantly lower than those in the control group and blank shRNA group(P<0.05).Conclusion Silencing p62 gene can inhibit the proliferation of cervical cancer Hela cells,promote cell apoptosis and increase the sensitivity to cisplatin.
作者
娄欢
马金平
武明莉
杨小风
LOU Huan;MA Jinping;WU Mingli;YANG Xiaofeng(Department of Obstetrics and Gynecology,Zhengzhou Central Hospital,Zhengzhou University,Zhengzhou,Henan,450000)
出处
《实验与检验医学》
CAS
2019年第2期172-174,218,共4页
Experimental and Laboratory Medicine
基金
河南省高等学校重点科研项目
15A321139
