摘要
冬虫夏草是一味传统名贵中药材,甾醇类成分是其重要的活性成分之一。为了探讨冬虫夏草不同生长阶段甾醇类成分的变化,本研究采用高效液相-蒸发光散射检测器技术对冬虫夏草5个不同阶段样品(冬虫夏草发酵菌丝体、僵虫、1cm子座冬虫夏草,4–6cm子座冬虫夏草,孢子弹射冬虫夏草)和对照样品(蝙蝠蛾幼虫)中麦角甾醇、胆甾醇和谷甾醇的含量进行了分析。结果显示4–6cm子座冬虫夏草样品总甾醇含量较高,该结果为冬虫夏草采收期的选择提供了参考。
Sterol is one of the important active substances in Chinese cordyceps,Ophiocordyceps sinensis.In this study,HPLC-ELSD was applied to detect three main sterols,ergosterol,cholesterol and sitosterol,in five different development stages of Chinese cordyceps,namely mycelium,ossified larva,1 cm stroma stage,4–6 cm stroma stage and sporulation stage.Uninfected Hepialus larva sample was set up as experimental control.The results showed that content of total sterols in 4–6 cm stroma stage was the highest.This result provides reference for harvesting Chinese cordyceps timely.
作者
钱正明
孙敏甜
李文庆
李光荣
李文佳
QIAN Zheng-Ming;SUN Min-Tian;LI Wen-Qing;LI Guang-Rong;LI Wen-Jia(Key Laboratory of State Administration of Traditional Chinese Medicine,Sunshine Lake Pharma Co.,LTD,Dongguan,Guangdong,523850,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2019年第4期539-544,共6页
Mycosystema
基金
国家工信部中药材提升和保障领域项目~~
关键词
麦角甾醇
胆甾醇
谷甾醇
高效液相色谱法
ergosterol
cholesterol
sitosterol
high performance liquid chromatography(HPLC)
