摘要
观察中药复方重肌灵对EAMG大鼠血清IL 4、IFN γ调节作用。方法 :采用N2 AchR加等量福氏完全佐剂 ,多次免疫Lewis大鼠 ,复制EAMG模型 ,并对模型进行评价。动物随机分为正常组、佐剂组、模型组、地塞米松组和重肌灵组。采用ELISA法观察各组动物血清AchRab、IL 4、IFN γ水平。结果 :重肌灵、地塞米松组大鼠血清AchRab滴度、IL 4、IFN γ水平明显降低 ,与模型组比较 ,有非常显著性差异 (P <0 0 1)。但两治疗组之间无显著性差异 (P >0 0 5 )。结论 :重肌灵具有良好的防治EAMG效果 ,可能是通过下调血清IL 4、IFN γ ,抑制AchRab产生 ,而起到治疗作用。
To observe immunoregulation effect of Zhong jiling to the level of IL 4 and TFN γ in Serum of EAMG rats.Method:EAMG model in Lewis rat as induced by immunizing the rats for 3 times with the mixture of N 2AchR and CAF,and randomly divided into 5 groups.The level of IL 4 and IFN γ in serum in diffent groups was observed by ELISA method.Results the level of IL 4 and TFN γ in serum in Zhong jiling group was obviously lower than that in model group.There was no significant difference ( P<0 01 ).Conclusions:The mechanism of Zhong jiling to prevent and treat EAMG might be explained by lowering the level of IL 4 and IFN γ to restrain specific immune reaction induced by N 2AchR antigen.
出处
《中国实验方剂学杂志》
CAS
2003年第2期35-37,共3页
Chinese Journal of Experimental Traditional Medical Formulae
