摘要
目的 :观察人骨髓间充质干细胞 (mesenchymalstemcells,MSCs)在体外培养的生物学特性和作为组织工程的种子细胞的可能性 .方法 :将人骨髓MSCs自骨髓中分离、纯化和培养 ,观察原代和传代培养的增殖及生长特征 .结果 :原代培养及传代培养显示 ,在体外培养条件下人骨髓MSCS有活跃的增殖能力 .结论 :体外获得的人骨髓MSCs的生物年龄较为年轻 。
AIM: To investigate the biological character of human bone marrow derived mesenchymal stem cells (MSCs) in vitro and to observe the feasibility of using MSCs as seed cells in tissue engineering. METHODS: Human MSCs were isolated from bone marrow and purified by centrifuge and cultured in vitro . The proliferation and growth characteristics of human bone marrow derived MSCs were observed in primary and passage culture. RESULTS: Human bone marrow derived MSCs showed active proliferation capacity in vitro in primary and passage cultures. CONCLUSION: Cells derived from human bone marrow derived MSCs have been successfully cultured, which have relatively young biological age and can be used as seed cells for tissue engineering.
出处
《第四军医大学学报》
北大核心
2003年第4期351-353,共3页
Journal of the Fourth Military Medical University
基金
第四军医大学科技创新工程基金资助项目 (CX99A0 1 8)
关键词
骨髓间充质干细胞
体外培养
生物特性
观察
mesenchymal stem cells
bone marrow
seed cells
biomedical engineering
