摘要
目的建立高效液相色谱法同时测定苦参碱杀虫剂中苦参碱、氧化苦参碱、槐定碱和槐果碱含量的方法。方法高效液相色谱法测定生物碱,色谱条件:色谱柱,Wondasil C18(4.6mm×250mm,5μm);流动相,乙腈-0.05mol/L磷酸二氢钾水溶液(三乙胺2%,15∶85,v/v);流速,1.0ml/min,检测波长:205nm。结果苦参碱、氧化苦参碱、槐定碱和槐果碱分别在0.076~0.5,0.084~0.4,0.12~0.44,0.20~0.49μg/ml浓度范围内呈良好线性关系(R2>0.998),含量分别为:0.70%,0.28%,0.60%,0.01%。结论本实验建立的HPLC法能快速同时测定苦参碱杀虫剂中4种生物碱的含量,可用于快速测定药品或环境中的生物碱。
Objective To establish a method for the simultaneous determination of the content of matrine,oxymatrine,sophoridine and sophocarpine in matrine pesticides by HPLC. Methods The alkaloids was determined by HPLC. The chromatographic conditions were established with WondasilC18( 4. 6mm × 250 mm,5μm),mobile phase of acetonitrile -0. 05mol/L potassium dihydrogen phosphate( 2% triethylamine,15: 85,v/v),flow rate of 1. 0 mL/min and detection wavelength of 205 nm. Results The matrine,oxymatrine,sophoridine and sophocarpine has good linearity relation( R2 > 0. 999) at the range of 0. 076 ~ 0. 50、0. 084 ~ 0. 47、0. 12 ~ 0. 44、0. 20 ~ 0. 49μg/mL,respectively. The contents were 0. 70%,0. 28%,0. 60%,and 0. 01%,respectively. Conclusion The four alkaloids were determined simultaneously by HPLC method in matrine pesticides,which can be used to determine the alkaloids content in drug or environment.
出处
《济宁医学院学报》
2017年第4期268-270,共3页
Journal of Jining Medical University
基金
国家自然科学基金项目(21405162)
