摘要
选取8种玉米基因型的幼胚为外植体,以改良MS培养基为基本培养基,对基因型、幼胚大小、培养基激素配比等影响因素进行分析,研究玉米杂交种高效再生体系。结果表明:各试验材料均能诱导出一定数量的愈伤组织,但不同基因型愈伤组织的诱导率、愈伤组织的胚性诱导和保持能力存在差异,具有显著的基因型依赖性。在本试验条件下,杂交种登海11号愈伤组织的诱导率、胚性性状及其保持能力均十分优秀,是一个理想的供试材料。此外,一些重要影响因素的优化,如选用1~2 mm长的幼胚,在诱导培养基中添中2~3mg/L 2,4-D以及分化培养基中添加0.5 mg/L 6-BA,可分别显著提高登海11号愈伤组织的诱导率和分化率。
Using eight maize lines as target genotypes, a highly efficient regeneration system was developed with MS being used as basal culture medium. Effects of genotypes, immature embryos size and hormone concentrations on the induction of embryogenic callus were investigated in this work. The results showed that all experimental maize materials could be induced to form callus. However, the rates of callus induction, embryogenic callus induction and retention were different among different genotypes. Under our experimental conditions, the best induction and subculture of embryogenic callus was obtained form 'Denghai No.11' maize hybrid. Furthermore, some important factors were optimized. The results showed that the immature embryos of1.0-2.0 mm in length in DH11 were optimal explants. Addition of 2-3 mg/L 2,4-D in induction medium significantly stimulated embryogenic callus of DH11. And the differentiation medium supplemented with0.5mg/L 6-BA had very great promoting effect on inducing differentiation.
出处
《吉林农业科学》
CSCD
北大核心
2014年第3期1-4,共4页
Journal of Jilin Agricultural Sciences
关键词
玉米
再生体系
杂交种
基因型
Maize
Regeneration system
Hybrid
Genotype
