摘要
目的 分析鼠与人粘蛋白 1(MUC1)的同源性位点。方法 用抗人类MUC1单克隆抗体GP1.4、CA15 3,通过细胞ELISA和Westernblot鉴定鼠Lewis肺癌细胞表面MUC1分子的表达 ,并用蛋白分析软件分析鼠与人MUC1分子的同源性。结果 抗人类MUC1单克隆抗体GP1.4和CA15 3与Lewis肺癌细胞表面MUC1存在交叉反应 ;鼠与人MUC1分子约具有 4 9.1%的同源性。结论 鼠与人MUC1分子具有一定的同源性 ,可用鼠Lewis肺癌细胞作荷瘤鼠研究人类MUC1肿瘤疫苗 ;
Objective To analyse the homology of mucin 1(MUC1) on mou se cells and human cells.Methods Identification of MUC1 express ion on Lewis lung cancer cell and human cell by ELISA and Western blot. Homolog y analysis of MUC1 on mouse cells and human cells were studied by software of pr otein analysis. Results There are cross reactions between anti human McAb GP1.4,anti human McAb CA15 3 and MUC1 on Lewis lung cancer cell ;There is about 49.1% similarity on MUC1 between mouse and human.Conclu sions There is homology between MUC1 of mouse and human. We can make an animal model with Lewis lung cancer cells for the study of MUC1 tumour vaccine. MUC1 on mouse cells can be used for human tumour vaccine. [
出处
《免疫学杂志》
CAS
CSCD
北大核心
2003年第2期83-85,共3页
Immunological Journal
基金
国家自然科学基金资助项目 (396 70 6 86 )
