摘要
目的:观察人脐带间充质干细胞移植对大鼠急性肝损伤的修复作用。方法:健康雄性SD大鼠随机分为3组:正常对照组A,CCl4造模+生理盐水组B及CCl4造模+hUCMSCs组C。应用病理组织学、肝功能测定方法观察大鼠肝脏结构及肝功能变化情况。结果:hUCMSCs移植后,C组与B组比较,肝损伤大鼠的ALT(P<0.05)及AST(P<0.01)水平明显降低,hUCMSCs移植可降低大鼠肝脏损伤的ALT及AST水平。C组肝脏组织学更快恢复,细胞移植7d后肝脏结构基本恢复正常,而B组仍可见脂肪滴伴有炎性细胞浸润。结论:大鼠移植hUCMSCs后可以在一定程度上改善肝功能及修复受损的肝组织。
Objective:To investigate the treatment effect hUCMSCs on rats acute liver damage model caused by CCl4.Methods:Rats were divided into 3 groups:normal control,CCl4 and CCl4 plus hUCMSCs.The rats were sampled at the appointed times and various target markers were determined as follows:HE staining,the index changes of liver’s enzynmlogy to evaluate the change of hepatic histological damage.Result:The levels of ALT（P<0.05）and AST（P<0.01）were obviously increased in B gronp than in A group,but reduced in C group than in B group.Conclusion:These findings indicate that transplantation of human umbilical cord mesenchymal stem cells can repair the injured liver function and repair injured liver tissues after acute liver injury in rats to some extent.
出处
《健康之路》
2017年第10期1-2,共2页
Health Way
关键词
脐带间充质干细胞
无血清培养基
移植
急性肝损伤
Human umbilical cord mesenchymal stem cells
Serum-free medium
Transplantation
Acute hepatic injury
