摘要
传统儿童肿瘤早期诊断方法未对激光荧光特征光谱进行分析,有效性差,为此提出基于激光荧光光谱技术的儿童肿瘤早期诊断方法,采用可调谐激光器发出激光耦合光纤束,照射到人体组织上产生自体荧光,荧光信息传送到计算机进行解析,为避免远紫外光对儿童皮肤的损害,选择365 nm～415 nm紫外波段的激光激发自体荧光。通过激光诱导荧光特征光谱分离算法计算RBFN权值,将激光诱导荧光特征光谱中的各个光谱变量分离出来,利用特征光谱分析算法获得各个光谱变量的值,分析这些光谱变量值和人体组织液中的荧光因子实现儿童肿瘤的早期诊断。实验结果表明,本文方法可实现儿童肿瘤早期精确诊断。
The early diagnosis method of childhood tumors based on confocal laser microscope and digestive tract was not analyzed and the effectiveness was poor.An early diagnosis method for childhood tumors based on laser fluorescence spectroscopy is proposed.A laser coupled fiber beam is emitted by a tunable laser,which irradiates into human tissues to produce an autologous fluorescence,and fluorescence information is transmitted to the computer for analysis.In order to avoid the damage of ultraviolet light to the skin of children,laser excitation self-fluorescence in the ultraviolet band of 365 nm to 415 nm was selected.The weight of RBFN is calculated by the laser induced fluorescence characteristic spectral separation algorithm.Each spectral variable in the laser induced fluorescence characteristic spectrum is separated and the value of each spectral variable is obtained by using the characteristic spectral analysis algorithm.These spectral variables and fluorescence factors in human tissue fluid are analyzed to realize early diagnosis of childhood tumors.The experimental results show that the concentration of humic acid solution of 3.9 mg/l obtained by the proposed method is very close to the actual concentration of 4 mg/l,indicating that the proposed method can accurately detect abnormal tissue fluids in children.At the same time,the best excitation wavelength of 380 nm with the maximum fluorescence difference can be used for early diagnosis of childhood tumors.
作者
金仙梅
吴双
郝杰
JIN Xianmei;WU Shuang;HAO Jie(Hospital of Jilin University,Changchun Jilin 130021,China;China-Japan Union Hospital of Jilin University,Changchun 130021,China;Jilin Cancer Hospital,Changchun Jilin 130021,China)
出处
《激光杂志》
北大核心
2019年第5期192-196,共5页
Laser Journal
基金
吉林大学第一医院青年发展基金(No.JDYY82017035)
关键词
激光
荧光光谱
儿童肿瘤
早期诊断
荧光因子
laser
fluorescence spectroscopy
children’s tumors
early diagnosis
RBFN
fluorescent factor
