摘要
目的 :探讨卵巢癌患者血清 CA12 5和机体免疫功能的表达特征。方法 :采用流式细胞术检测患者外周血细胞 CD+ 3 、CD+ 4 、CD+ 8、CD+ 4 / CD+ 8、CD+ 1 9和 CD- 3 / CD+ ( 1 6 + 56 ) (NK细胞 )的百分率 ,采用酶联免疫法检测患者血清 CA12 5的浓度。结果 :卵巢癌患者血清 CA12 5浓度显著高于正常对照组 (P<0 .0 1) ;患者 CD+ 3 、CD+ 4 、NK+和 CD+ 1 9细胞均显著低于正常对照 (P<0 .0 5或 P<0 .0 1)。随着患者血清 CA12 5浓度的增加 ,CD+ 3 、CD+ 4 、NK+ 细胞均逐渐降低 ,并呈显著的负相关 (相关系数分别为 r1 =- 0 .95 9P<0 .0 1,r2 =- 0 .932 P<0 .0 5 ,r3=- 0 .912 P<0 .0 5 )。随着临床分期的增加 ,CA 12 5浓度逐渐增加 ,而和 CD+ 3 、CD+ 4 和 NK+ 细胞则逐渐降低 ;伴肿瘤转移患者的 CA12 5浓度显著高于未转移者 (P<0 .0 1) ,而 CD+ 3 、CD+ 4 和 NK+细胞却显著低于未转移者 (P<0 .0 5 )。结论 :卵巢癌能引起患者血清 CA12 5表达率明显上调 ,而使机体免疫功能显著下降。 CA12 5的表达与患者机体免疫状态之间以及它们与临床生物学行为之间都有一定关系。
Objective:To investigate the characteristic of CA125 expression level and immunological state of patients with ovarian cancer.Methods:The concentration of serum CA125 and the percentage of CD+ 3,CD+ 4,CD+ 8,CD+ 19,CD- 3/CD+ (16+56)NK+ cells and CD 4/CD 8 were measured in peripheral blood cells from 33 patients with ovarian cancer by ELISA and flow cytometry.Results:The concertration of serum CA125 in patients with ovarian cancer was significantly higher than that of normal control(P<0.01). The percentage of CD+ 3,CD+ 4 and NK+ cells of patients with ovarian cancer were significantly lower than those of normal control (P<0.01 or P<0.05). The correlation analysis showed that the concentration of serum CA125 were negatively correlated to CD+ 3, CD+ 4 and NK+ cells percentage (r 1=-0.959, P<0.01; r 2=-0.932, P<0.05; r 3=-0.912, P<0.05 ). The gradually increasing of serum CA125 concentration and the gradually decreasing of CD+ 3, CD+ 4, and NK+ cells percentage were with increasing of clinical stages on patients with ovarian cancer. Serum CA125 concertration of patients with metastasis was higher than that of patients with non-metastasis (P<0.01). The percentage of CD+ 3, CD+ 4 and NK+ cells of patients with metastasis were lower than those of patients with non-metastasis (P<0.05).Conclusion:Ovarian cancer may induce significant increasing of the concentration of serum CA125 and may induce significant decreasing of percentage of CD 3,CD 4 and NK cells. There is a very colosed relationship between the concentration of serum CA125 and the immunological state of patients as well as the clinical biological behavious of patients with ovarian cancer.
出处
《肿瘤研究与临床》
CAS
2003年第1期29-32,共4页
Cancer Research and Clinic
