摘要
大鼠70只,腹腔注射二乙亚硝氨,饲以含α-乙酸氨基芴的饲料并行2/3肝切除,大鼠肝内迅速出现增生肝细胞灶,组化染色改变:γ-GT阳性,ATP酶、G6P酶阳性减弱或阴性,禁食后PAS阳性.连续切片中4种组化染色检出的增生肝细胞灶数以γ-GT染色最多,PAS染色最少。增生肝细胞灶AKP染色为膜强阳性,胞浆弱阳性。γ-GT与AKP检出的增生灶数无显著差异,提示二者可作为增生肝细胞灶的标志酶。
In order to observe the altered hepatocytic foci or nodules in early stages' experimental hepatocarcinogenesis in rats, we adopted five histochemical methods, i. e. r-GT, ATPase, G6Pase, fasting PAS and AKP staining. The altered foci or nodules developed in response to the intraperitoneal injection of diethylnitrosamine (DEN), feeding of 2-acetoaminoflurene (2-AAF) and 2/3 hepatectomy, and were identified as r-GT positive, ATPase negative, G6Pase negative, fasting PAS positive and AKP positive reactions in our study. Statistical analysis showed that in serial sections the number of the altered foci and nodules estimated by the first four stainings was significantly different from one another (P<0. 05 to<0. 001). The number assessed by r-GT positive staining ranked the first (largest), and by PAS the last (smallest). We found that the positive AKP reaction of the altered foci or nodules was stronger than that of extra-focal or nodular tissue. And the number of AKP positive foci and nodules was similar to that of r-GT positive foci and nodules in serial sections (P>0. 05). This suggested that as a marker of altered foci or nodules, AKP staining was as sensitive as r-GT staining.
关键词
肝肿瘤
病理学
化学诱导
liver neoplasms
chemical induction
early stage pathology
histochemical staining
