摘要
根据猪链球菌 2型的溶血素基因合成了一对可扩增长度为 14 96bp目的片段的引物 ,建立了检测溶血素基因的PCR方法。该方法对猪链球菌 2型参考株SS2 N株的检测结果为阳性 ,对马链球菌兽疫亚种 (C群 )参考株ATCC3 5 2 46、分离株SESZ Y、SESZ T、SESZ C和猪葡萄球菌、大肠埃希氏菌的检测结果均为阴性 ;用EcoRⅠ内切酶进行酶切 ,获得了与预期结果一致的 863bp和 63 3bp的 2个片段。经对SS2 1株的PCR产物进行测序及序列分析 ,表明其与 193 3株、P1/ 7株的核苷酸同源性分别为 99.7%和 10 0 %。
A PCR assay for the rapid and specificdetection of suilysis geneof Streptococcus suis type 2 was developed. The PCR primers based on the sly gene of S.suis type 2 can extend a 1 496 bp PCR product. The detection specificity of the PCR method was confirmed with PCR assay of SS 2 N strains, S.equisimil is subsp zooepidemicus (ATCC 35246, SESZ Y, SESZ T and SESZ C), Streptococcus hyicus and Escherichia coli. Being digested with EcoRⅠ , the PCR product can produce a 863 bp and a 633 bp DNA fragment as expected. All of 7 strains of S.suis type 2 isolated from diseased pigs were positive by PCR. The PCR product from SS 2 1 strain was sequenced and nucleotide sequence analysis revealed that the sly gene from strain SS 2 1 had a high homology to strains 1933(99.7%) and P1/7(100%).
出处
《中国兽医科技》
CSCD
北大核心
2002年第11期11-14,共4页
Chinese Journal of Veterinary Science and Technology
基金
国家"973"资助项目 (G1 9990 1 1 90 0 )
江苏省攻关项目(BE2 0 0 1 372 )
