摘要
目的:进一步改进龙胆泻肝丸中栀子苷的HPLC含量测定方法。方法:用Shimadzu C18柱,流动相为异丙醇-醋酸乙酯-水(1∶0.8∶92.2),在240nm波长处检测。结果:栀子苷线性范围为:0.1996~3.9920g,r=0.9999, 平均加样回收率为99.3%,RSD=1.9%(n=5)。结论:建立的栀子苷含量测定方法准确灵敏、稳定可靠,可用于龙胆泻肝丸的质量控制。
Objective:To establish a HPLC method for determination of Geniposide in Longdan Xiegan Wan. Method:Shimadzu C18 column was used. The mobile phase was iso-propyl alcohol-ethyl acetate-water (1∶0.8∶92.2). The detecting wavelength was 240 nm. Results: The detection ranges were 0.1996~3.9920g. The average recovery of Geniposide was 99.3%, RSD=1.9% (n=5). Conclusion: This HPLC method of Geniposide, which was convenient, rapid and accurate, could be used for quality control in the production of Longdan Xiegan Wan.
出处
《中国中医药信息杂志》
CAS
CSCD
2003年第1期29-30,共2页
Chinese Journal of Information on Traditional Chinese Medicine