摘要
目的 从信号转导这一层次探索青春型双歧杆菌的抑瘤途径。方法 以大肠癌裸鼠移植瘤为动物模型,用膜结合法、激光共聚焦显微镜和免疫组织化学分别测定了大肠癌组织蛋白酪氨酸激酶(PTK)、蛋白激酶C(PKC)α、βI、βⅡ、γ、ε和ξ的活性以及核因子(NF)-κB的活化状况。结果双歧杆菌注射组大肠癌组织PTK的活性、PKCα和βⅡ的平均荧光强度以及NF-κB的阳性细胞密度均明显低于肿瘤对照组(P<0.01),而PKCβI、γ、ε和ξ的平均荧光强度在两组间差异无显著性(P>0.05)。结论青春型双歧杆菌体内能显著降低大肠癌PTK和PKCα、βⅡ的活性,同时也能抑制其NF-κB的活化。
Objective To explore the antitumor pathway of bifidobacteria adolescentis in view of the signal transduction. Methods The activity of PTK, PKCα, βI , βII , γ, ε, ξ and the active station of NF-κB in large bowel carcinoma tissues was detected by using membraneous conjugated method, laser scanning confocal microscopy and immuriohistochemistry respectively in the animal model of nude mouse transplantation tumors of large bowel carcinoma. Results The PTK activity, average fluorescent intensity of PKCa, βII and positive cellular density of NF-κB in large bowel carcinoma tissues in bifidobacterium-treated group was markedly lower than in tumor control group ( P < 0. 01). There was no significant difference in average fluorescent intensity of PKCβI ,γ, ε and ξ between the two groups( P >0. 05). Conclusion Bifidobacteria adolescentis could significantly decrease the activity of PTK, PKCa, βII and inhibit the NF-κB activition in large bowel carcinoma in vivo.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2003年第2期134-136,共3页
Chinese Journal of Experimental Surgery
基金
广东省自然科学基金资助项目(994066)
