摘要
通过比较厚血膜涂片镜检法和PCR检测法在筛选培养恶性疟原虫单克隆虫株中的优缺点,结果显示,借助带显示屏显微镜的应用,厚血膜涂片镜检法在阳性检出率方面跟PCR检测法基本持平,在操作复杂性、操作时间以及成本等方面有一定优势,值得相关实验室重新采用这种经典的检测方法.
This study compared the advantages between the thick blood smear examination and PCR detection method in screening the monoclonal strains from cultured Plasmodium falciparum.The results showed that,with the application of microscope which equipped with a display screen,the positive detection rate by thick blood smear examination was almost equal to that of PCR method,and thick blood smear examination also exhibited more advantages,including operating process,operating time and cost.It was worthy for the relevant lab to reuse this classic method.
出处
《湖州师范学院学报》
2016年第8期96-99,共4页
Journal of Huzhou University
基金
浙江省自然科学基金(LY15H190007)
关键词
恶性疟原虫
单克隆筛选
厚血膜
PCR
Plasmodium falciparum
monoclonal strain screen
thick blood smear
PCR
