摘要
OBJECTIVE: To study Chlamydia pneumoniae (C. pneumoniae) infection in 110 patients with respiratory tract infection admitted to our hospital from January to December 1995 in Nanjing. METHODS: Sputum and throat swab specimens were taken and C. pneumoniae DNA was detected by using polymerase chain reaction (PCR) with the HM-1-HR-1 primer pair. At the same time, serum samples were taken and immunoglobulin G and M (IgG and IgM) fractions of antibodies to C. pneumoniae were studied by microimmunofluorescence test. RESULTS: Prevalence of specific IgG was 70% in patients with respiratory tract infection. Seventeen patients (15.5%) were serologically diagnosed as having recent C. pneumoniae infections and 12 patients (10.9%) had positive PCR in sputum and/or swab specimens. The total positive rate was 22.7% (25/110) detected by PCR combined with serological tests. Acute infection of C. pneumoniae was common in patients with asthma (57.1%), pneumonia (35.0%), COPD (25.9%) and bronchitis (25.0%). Clinical features between C. pneumoniae infection and non-C. pneumonia infection showed no significant differences. CONCLUSIONS: Chlamydia pneumoniae is an important pathogen that causes infection of the human respiratory tract and attention should be drawn to this special illness.
目的 了解成人呼吸道感染患者急性肺炎衣原体感染的发病率及临床特征。方法 呼吸道感染住院患者 110例 ,同时采集痰和咽拭子标本应用聚合酶链式反应 (PCR)检测肺炎衣原体DNA及采取静脉血检测肺炎衣原体IgG和IgM抗体。结果 本组呼吸道感染患者特异性IgG抗体的阳性率为 70 %。 17例 (15 5 %)有肺炎衣原体近期感染的急性抗体 ,12例 (10 9%)痰和 (或 )咽拭子肺炎衣原体PCR检测结果阳性 ,联合应用两种方法的阳性率为 2 2 7%(2 5 /110例 )。肺炎衣原体急性感染以支气管哮喘急性发作、肺炎、COPD急性加重和急性支气管炎患者多见 (分别为5 7 1%、35 0 %、2 5 9%和 2 5 0 %) ,其临床表现无特征性。结论 本组成人呼吸道感染患者肺炎衣原体急性感染的发病率较高 ,提示肺炎衣原体是呼吸道感染的重要致病原。
