摘要
将已在察氏液体培养基中培养 3 d的强毒性 (V44 )或弱毒性 (V 6 4)大丽轮枝菌接种到新鲜的相同培养基中 ,加入棉花黄萎病感病品种 (S)或抗病品种 (R)的悬浮培养细胞 (或其细胞匀浆 )。培养 4d后收获真菌孢子 ,用于致萎力测定 ,收获菌丝体细胞壁用于制备真菌寡糖素 (H,来自 V44或 L,来自 V6 4) ,然后用该寡糖素诱导悬浮培养的棉花细胞 (S或 R)。棉花细胞的过氧化物酶 (POD)同工酶结果表明 :(1)所有寡糖素均诱导棉花细胞产生新的过氧化物酶 ;(2 )感病品种豫棉 6号的活组培细胞 S(或其细胞匀浆 )显著影响真菌 ,用生长过程中接触该活细胞 (或其细胞匀浆 )的真菌所制备的寡糖素再诱导该活细胞 (即 S→ L (H )→ S)时 ,棉花细胞在 36 h的 POD同工酶谱与其对照 ,即 L(H)→ S比较 ,酶带增减变化显著。致萎力测定结果表明感病品种的活细胞提高了病原毒性和致病力 ;(3)耐病 (豫棉 8号 )或抗病品种 (中棉 12号 )的活组培细胞 R(或其细胞匀浆 )对真菌的影响不同于感病品种。 R→ L(H )→ R在 36 h的 POD同工酶谱与 L (H)→ R比 ,酶带没有增减变化。但是在 H→ R或 H→ S中于 9h和 2 4h出现的酶带在 R→ H→ R或 R→ H→ S中分别提前到 6 h和 9h,致萎力测定结果表明抗病品种细胞对病原毒性影响很小。
The living cell culture of cotton or its homogenates from V.dahliae-susceptible variety (S) or resistant variety (R) were added into the Czapeks liquid media which had been inoculated 3 d with the higher virulent strain(V44) and lower virulent strain(V64) of V.dahliae respectively. After 4 d additional incubation, the spore of these fungus were collected each to evaluate their wilting or virulence to cotton plants, and the cell wall of the hypha were used to prepare oligosaccharins(H,from V44 and L, from V64)which were as elicitors to induce the cell cultures of cotton.(S or R) The PAGE spectrums of POD isozymes from cotton cells showed that (1) all oligosaccharins induced cotton cells to produce new POD isozymes; (2)the living cotton cells or its homogenate from susceptible variety affected strongly the fungus. The cotton POD spectrum at 36 h after induction showed that in all the inducing systems, the most outstanding diversity is between S→L(H)→S and their control, i.e. L(H)→S, i.e. the oligosaccharins from the fungi contacted with the living cotton cells or its homogenate from susceptible variety induced the most strong change of POD isozymes in the living cotton cells from susceptible variety, as compared with the control, L(H)→S. The wilting result showed the living cotton cells from susceptible variety increased the virulence and pathogenicity of the fungi; (3)the impact of living cotton cells or its homogenate from resistant variety on the fungi is different from susceptible one. There is no change of enzyme bands, but the new enzymes occurred at 9 h in H→R and 24 h in H→S after induction were ahead to 6 h in R→H→R and 9 h in R→H→S. The wilting result showed the living cotton cells from resistant variety had little influence on the virulence and pathogenicity of the fungi.
出处
《武汉植物学研究》
CAS
CSCD
2002年第6期457-462,T006,共7页
Journal of Wuhan Botanical Research
