摘要
目的探讨人参皂苷Rg2对东莨菪碱诱导阿尔茨海默病小鼠学习记忆力的影响及可能的作用机制。方法昆明小鼠60只随机分为正常对照组(等体积生理盐水)、模型组(东莨菪碱4mg/kg)、多奈哌齐组(多奈哌齐3mg/kg+东莨菪碱4mg/kg)和人参皂苷Rg2低剂量组(人参皂苷Rg2 2.5 mg/kg+东莨菪碱4 mg/kg)、中剂量组(人参皂苷Rg25.0mg/kg+东莨菪碱4mg/kg)和高剂量组(人参皂苷Rg2 10.0mg/kg+东莨菪碱4mg/kg),每组10只。采用Morris水迷宫评价人参皂苷Rg2对小鼠学习记忆的影响;第11天测试结束处死小鼠,采用ELISA法检测4组小鼠海马和前脑皮层乙酰胆碱酯酶(acetyl cholinesterase,AChE)、乙酰胆碱转移酶(choline acetyl transferase,ChAT)活性和乙酰胆碱(acetylcholine,ACh)的含量。结果与模型组比较,人参皂苷Rg2低、中、高剂量组和多奈哌齐组小鼠定位航行实验逃避潜伏期缩短,小鼠穿越平台次数增加,有效区域滞留时间和游泳路程缩短(P<0.05);多奈哌齐组[(17.4±1.1)、(8.7±0.2)u/mg]、人参皂苷Rg2低剂量组[(21.6±0.3)、(8.6±0.3)u/mg]、中剂量组[(17.4±0.8)、(8.5±0.3)u/mg]、高剂量组[(15.8±0.4)、(8.0±0.3)u/mg]海马和前脑皮层中AChE活性均低于模型组[(23.2±1.2)、(10.2±0.8)u/mg](P<0.05);多奈哌齐组[(13.5±0.8)、(58.1±0.6)mg/g]、人参皂苷Rg2低剂量组[(13.0±0.8)、(47.7±1.0)mg/g]、中剂量组[(14.8±0.8)、(50.0±1.1)mg/g]、高剂量组[(14.5±0.8)、(53.2±2.5)mg/g]海马和前脑皮层中ACh水平均高于模型组[(8.6±0.4)、(35.9±1.2)mg/g](P<0.05);多奈哌齐组[(6.3±0.3)、(3.4±0.3)u/mg]、人参皂苷Rg2低剂量组[(4.7±0.4)、(3.0±0.2)u/mg]、中剂量组[(5.9±0.2)、(3.5±0.1)u/mg]、高剂量组[(6.2±0.6)、(3.3±0.2)u/mg]海马和前脑皮层中ChAT活性均高于模型组[(3.4±0.3)、(2.7±0.4)u/mg](P<0.05)。结论人参皂苷Rg2对东莨菪碱诱导的阿尔茨海默病模型小鼠的学习记忆功能有显著的改善作用,其机制可能与抑制小鼠海马和前脑�
Objective To investigate the influence of ginsenoside-Rg2 on scopolamine-induced learning and memory impairment in mice and its possible mechanism.Methods Sixty Kunming mice were randomly divided model group(4mg/kg scopolamine),donepezil group(3mg/kg donepezil+4mg/kg scopolamine),and low-dose group(2.5mg/kg ginsenoside-Rg2+4mg/kg scopolamine),medium-dose group(5.0 mg/kg ginsenoside Rg2+4 mg/kg scopolamine),high-dose group(10.0 mg/kg ginsenoside-Rg2+4 mg/kg scopolamine)and control group(equivalent normal saline),with 10 mice in each group.The influence of ginsenoside-Rg2 on scopolamine-induced learning and memory was assessed by Morris water maze test.The levels of acetyl cholinesterase(AChE),choline acetyl transferase(ChAT)and acetylcholine(ACh)were measured by ELISA.Results Compared with model group,low-,medium-and high-dose groups and donepezil group significantly shortened the positioning flight test escape latency,increased the number of crossing the platform,and shortened the effective area retention time and swimming distance(P<0.05).The levels of AChE in the hippocampus and forebrain cortex were significantly lower in donepezil group((17.4±1.1),(8.7±0.2)u/mg),low-dose group((21.6±1.1),(8.7±0.2)u/mg),medium-dose group((17.4±0.8),(8.5±0.3)u/mg),and high-dose group((15.8±0.4),(8.0±0.3)u/mg)than those in model group((23.2±1.2),(10.2±0.8)u/mg)(P<0.05).The levels of ACh in the hippocampus and forebrain cortex were significantly higher in donepezil group((13.5±0.8),(58.1±0.6)mg/g),low-dose group((13.0±0.8),(47.7±1.0)mg/g),medium-dose group((14.8±0.8),(50.0±1.1)mg/g),and high-dose group((14.5±0.8),(53.2±2.5)mg/g)than those in model group((8.6±0.4),(35.9±1.2)mg/g)(P<0.05).The activities of ChAT in the hippocampus and forebrain cortex were ignificantly higher in donepezil group((6.3±0.3),(3.4±0.3)u/mg),low-dose group((4.7±0.4),(3.0±0.2)u/mg),medium-dose group((5.9±0.2),(3.5±0.1)u/mg),and high-dose group((6.2±0.6),(3.3±0.2)u/mg)than those in model group((3.4±0.3),(2.7±0.4)u/mg)(P<0.05).C
出处
《中华实用诊断与治疗杂志》
2017年第5期444-447,共4页
Journal of Chinese Practical Diagnosis and Therapy
基金
武警后勤学院附属医院种子基金(FYM201519)
