摘要
目的:分析红细胞膜蛋白带4.9(EPB49)在结直肠癌组织中表达水平及与患者预后的关系。方法:选取2011年6月~2016年8月我院随访满5年的结直肠癌石蜡样本与25例新鲜组织样本,应用免疫组织化学(IHC)、免疫印迹法(westernblot)测定所有样本EPB49表达情况,检测EPB49过表达与干扰后Rac GTP酶激活蛋白1(Rac1-GTP)表达水平,验证EPB49与T淋巴细胞瘤侵袭转移诱导因子1(Tiam1)的相互作用,分析EPB49与分化程度、Dukes分期、转移及Rac1-GTP、Tiam1水平的相关性。结果:IHC技术及westernblot实验显示正常结直肠癌组织EPB49表达水平高于相配对的肿瘤组织;随分化程度、Dukes分期增加,EPB49表达水平下降;EPB49低表达组的转移率68.0%高于高表达组38.0%,而其3年生存率74.0%、5年生存率60.0%低于高表达组90.0%、80.0%;相关分析显示EPB49表达水平与分化程度、Dukes分期及Rac1-GTP、Tiam1表达水平呈负相关。结论:EPB49在结直肠癌组织中呈低表达状态,且与患者肿瘤分化程度、分期、转移及Rac1-GTP、Tiam 1表达水平呈负相关性。
Objective To analyze the expression level of erythrocyte membrane protein band 4.9(EPB49) in colorectal carcinoma and its relationship withprognosis. Methods Colorectal carcinoma paraffin samples and 25 cases of fresh tissue samples which were followed up for at least 5 years(from June 2011 to August 2016) in our hospital were studied, and the expression of EPB49 in all samples were detection by immunohistochemistry(IHC) and Western blot method. The overexpression of EPB49 and expression of Rac GTP activator protein1(Rac1-GTP) after interference were detected, and the interaction between EPB49 and T lymphocytoma invasion and metastasis inducing factor 1(Tiam1) was verified. The correlations between EPB49 and differentiation degree, Dukes stage, metastasis, Rac1-GTP and Tiam1 levels were analyzed. Results IHC and Westernblot showed that expression level of EPB49 in normal colorectal carcinoma tissues was higher than that in corresponding tumor tissues, and it decreased with the increase of degree of differentiation and Dukes stage; The metastasis rate of EPB49 low expression group(68.0%) was higher than that of high expression group(38.0%), while the 3-year and 5-year survival rates(74.0%, 60.0%) were lower than those in high expression group(90.0%, 80.0%); Correlation Analysis showed that the expression level of EPB49 was negatively correlated with the degree of differentiation, Dukes stage, expression levels of Rac1-GTP and Tiam1. Conclusion EPB49 is lowly expressed in colorectal carcinoma, and itis negatively correlated with the degree of tumor differentiation, stage, metastasis, expression levels of Rac1-GTP and Tiam 1.
出处
《湖南师范大学学报（医学版）》
2018年第1期94-98,共5页
Journal of Hunan Normal University(Medical Sciences)
