摘要
目的:建立中风通络丸中丹参酮ⅡA和丹酚酸B的含量同时测定法。方法:色谱柱为依利特Hypersil-ODS(4.6 mm×250 mm,5μm);流动相为乙腈-0.5%甲酸溶液;梯度洗脱:0~15 min,乙腈10%~20%;15~25 min,乙腈20%~50%;25~35 min,乙腈50%~60%;35~40 min,乙腈60%~95%,;流速为1.0 mL·min-1;检测波长:丹参酮ⅡA为270 nm,丹酚酸B为286 nm;柱温为30℃。结果:丹参酮ⅡA的线性方程为:Y=4×106X+175439(r=0.9998),线性范围为0.1861~0.4839μg,加样回收率为99.15%,RSD为1.20%;丹酚酸B的线性方程为:Y=2×106X+36551(r=0.9998),线性范围为0.6258~2.1902μg,加样回收率为100.19%,RSD为1.44%。结论:本方法操作简便,结果可靠,重现性好,可以用于中风通络丸的质量控制。
Objective To propose an HPLC quantitative method for simultaneously determining tanshinoneⅡAand salvianolic acid B in Zhongfeng Tongluo Pill. Methods Analysis were carried out using an Hypersil-ODS( 4.6mm ×250mm,5μm),the mobile phase consisted of acetonitrile and 0.5% formic acid in water with gradient elution( ACN- 0. 5% formic acid: H2O: 0 ~ 15 min,10% ~ 20%; 15 ~ 25 min,20% ~ 50%; 25 ~ 35 min,50% ~ 60%; 35 ~ 40 min,60% ~ 95%),and the flow rate was 1. 0 mL /min. The UV detection wavelength was at 270 and 286 nm for tanshinoneⅡAand salvianolic acid B respectively. The column oven temperature was at 30℃. Results TanshinoneⅡAand salvianolic acid B were separated and remained in good linearity in the range of0. 18613 ~ 0. 48394 μg and 0. 62578 ~ 2. 1902 μg with the linear equationof Y = 4 × 106X + 175439( r = 0. 9998)and Y = 2 × 106X + 36551( r = 0. 9998) respectively. The average recoveries were 99. 15% and 100. 19%,and the RSD were 1. 20% and 1. 44%. Conclusion The method appeared to be precise,reliable and simple,and can be used to control the quality of Zhongfeng Tongluo Pill.
出处
《湖南师范大学学报（医学版）》
2014年第2期91-93,共3页
Journal of Hunan Normal University(Medical Sciences)
