摘要
目的:探讨腹部不同强度的手术刺激对肺部的影响。方法:将100只SD雄性大鼠随机分为5组,每组20只。分别为麻醉对照组(A组),腹部皮肤2 cm切口组(B组),腹部皮肤+肌肉2 cm切口组(C组),腹部皮肤+肌肉+腹膜2 cm切口组(D组),腹部皮肤+肌肉+腹膜2 cm切口+腹腔探查2 min+牵拉肠管组(E组)。经大鼠尾静脉注射丙泊酚10 mg/kg诱导,术中静脉泵注丙泊酚35~45 mg/(kg.h),维持麻醉深度在适度镇静而无明显镇痛作用的水平。根据术后取标本的时间不同每组各分为6 h,1 d,3 d,7 d组4个亚组,每亚组5只。按分组取大鼠肺组织,采用免疫组织化学方法测定各组大鼠肺组织肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和肺表面活性物质相关蛋白A(surfactant protein-A,SP-A)的表达水平,并进行比较分析。结果:术后6 h,3 d,7 d,5组大鼠肺组织TNF-α的表达差异无统计学意义(P>0.05)。术后1 d,D和E组大鼠肺组织TNF-α的表达明显高于A组(P<0.05)。术后1和3 d大鼠肺组织TNF-α的表达E组>D组>C组>B组,B^E组各组间比较,差异无统计学意义(P>0.05)。术后6 h,3 d,7 d,5组大鼠肺组织SP-A的表达比较,差异无统计学意义(P>0.05)。术后1 d,D和E组大鼠肺组织SP-A的表达明显高于A组(P<0.05),D和E组组间比较,差异无统计学意义(P>0.05)。结论:腹部手术刺激强度增加对大鼠术后肺部影响增大,腹腔内手术对术后肺部的影响比非腹腔内手术大,但尚处于代偿范围内。
Objective: To determine the influence of different stimulation of abdominal operation on lung. Methods: We randomly divided 100 SD male rats into 5 groups(n=20): An anesthesia control group(Group A), an abdominal skin 2 cm incision group(Group B), an abdominal skin+ muscle 2 cm incision group(Group C), an abdominal skin+muscle+peritoneum 2 cm incision group(Group D), and an abdominal skin+muscle+peritoneum 2 cm incision+2 min laparoscopy in abdomen+pull exploration bowel group(Group E). Propofol was used for induction of anaesthesia by rat tail vein with 10 mg/kg, with 35–45 mg/(kg.h) during the operation. Anesthesia depth was maintained during moderate sedation without obvious analgesia action level. According to the postoperative specimens at different time, each group was divided into 4 sub groups, including 6-hour group, 1-day group, 3-day group, and 7-day group(n=5). Immunohistochemical method was used to examine tumor necrosis factor(TNF-α) and lung tissue lung surface active substances related proteins A(SP-A) of rats at different points after surgery. Results: There was no significant differences in the expression of TNF-α at 6 hours, 3 days, and 7 days after surgery in the 5 groups(P>0.05). The expression of TNF-α in Group D, and E was significantly higher than that in Group A at 1 day after surgery(P<0.05). Although the expression of TNF-α was GroupE > Group D > Group C > Group B at 1 day, 3 days after surgery, there was no significant difference among the 4 groups. There was no significant differences in the expression of SP-A at 6 hours, 3 days, and 7 days after surgery in the 5 groups(P>0.05). The expression of SP-A in Group D and E was significantly higher than that in Group A at 1 day after surgery(P<0.05). There was no significant difference between Group D and E(P>0.05). Conclusion: The greater the stimulant intensity of abdominal operation on rats, the more impact on postoperative lung. The postoperative pulmonary effect of intraperitoneal operation is greater than non-intraperitoneal oper
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2014年第9期883-888,共6页
Journal of Central South University ：Medical Science
关键词
腹部手术
肿瘤坏死因子Α
肺表面活性物质相关蛋白A
abdominal operation
tumor necrosis factor α
lung surface active substances related proteins A
