摘要
酸性脲酶产生菌Y2002L9经紫外线(UV)照射,亚硝基胍(NTG)处理,UV NTG复合诱变及培养条件优化后,酶活可达到2.63u/ml。在pH6.5～7.0、温度30～32℃、尿素浓度为10g/l时产酶活跃。当Ni+浓度为0.01%,Mn+浓度为0.001%～0.01%时对产脲酶有促进作用。在浓度为0.001%～0.01%范围内Co2+,Zn2+,Al3+对产脲酶没有影响,Pb2+,Fe3+,Ag+对产酶有抑制作用,Hg2+对产酶抑制作用较强。该菌株所产脲酶为胞内酶,经超声波破碎后,用pH6.5,浓度0.05mol/l柠檬酸缓冲液抽提,再用乙醇分级沉淀法可获得粗酶液。
The strain of high yielding urease is selected from the strains being treated in UV, NTG, and UVNTG,respectively. By optimum conditions studying of the composition of fermentation medium and some culture conditions, the strain urease yield could reach 2.63 u/ml. The strain producing urease is active under the condition of the primary pH 6.5-7.0, temperature 30-32℃,and urea concentration 10 g/ l. Mn2+(0.001%-0.01%) and Ni2+(0.01%) have promotion effect on producing urease.Co2+, Fe3+,Zn2+ and Al3+(0001%-0.01%) have no promotion effect, while Hg2+,Pb2+ and Ag+ have restraining. The strain produces urease inits cells. After the cells are disrupted in ultrasonic disintegrator at 17 Amp,the urease was extracted with citric acid buffer at 50 mmol/l and pH 6.5.
出处
《浙江农业学报》
CSCD
2002年第6期334-339,共6页
Acta Agriculturae Zhejiangensis
基金
浙江省自然科学基金资助项目(394160)
