摘要
目的探讨尿酸钠(monosodium urate,MSU)结晶体外刺激对多形核中性粒细胞(polymorphonuclear neutrophils,PMNs)、单核细胞细胞株THP-1、成纤维滑膜细胞(fibroblast-likes synoviocytes,FLSs)的活性及三种细胞趋化PMNs功能的影响。方法体外培养人PMNs、THP-1、FLSs,每种细胞各分为对照组和MSU组,并设无细胞的对照组和MSU组,对照组均为不含MSU结晶的培养液,MSU组为含终浓度为500 mg/L的MSU结晶培养液,所有细胞组于培养0、12、24、48 h后MTS法检测细胞的增殖,所有组别采用Transwell法观察培养24 h后对PMNs的趋化作用。结果培养12、24、48 h后,PMNs、THP-1的MSU组和对照组活性均随着时间的延长而增加,各时间点MSU组细胞活性均较对照组明显增高,比较差异具有统计学意义(P<0.01)。FLSs的MSU组在12、48 h细胞活性较对照组有明显降低(P<0.05),24 h时降低无明显差异(P>0.05)。培养24 h后,与对照组比较,PMNs和THP-1的MSU组对PMNs的趋化作用明显下降,差异有统计学意义(P<0.05),FLSs的MSU组对PMNs的趋化作用明显增加,差异具有统计学意义(P<0.05)。结论 MSU结晶在一定时间内,能明显促进PMNs、THP-1细胞活性增加,而对FLSs的活性则有抑制作用。MSU结晶刺激后PMNs、THP-1对PMNs的趋化下降,而FLSs对PMNs的趋化增加。
Objective To investigate the influence of monosodium urate crystals on vitality and chemotaxis to the polymorphonuclear neutrophils( PMNs),THP-1 cells and fibroblast-likes synoviocytes( FLSs) in vitro. Methods Human PMNs、THP-1 and FLSs were divided into control group and MSU group. Blank groups without cells were divided into control group and MSU group too. All control groups contained serum-free medium and MSU groups contained 500 mg / L monosodium urate crystals.At 0,12,24 and 48 hours,cell viability of the three kinds of cells were tested with MTS method. At 24 hours the number of chemotactic PMNs were tested by transwell chambers. Results At the time points of 12,24,48 h,the vitality of PMNs,THP-1 in two groups increased both along with the prolong of time,Compared with control group the vitality of PMNs,THP-1 in MSU groups increased significantly at the same time( P<0. 01),but the viability of FLSs decreased significantly in MSU group at 12,48 h( P<0. 05,P<0. 01),and had no significant difference at 24 h( P>0. 05). Compared with control group,PMNs and THP-1 in MSU group decreased chemotaxis to PMNs significantly( P<0. 05),but FLSs in MSU group increased chemotaxis to PMNs significantly after stimulation by MSU crystalsat 24 h( P< 0. 05). Conclusions Monosodium urate crystals could promote the vitality of PMNs,THP-1,but suppress the vitality of FLSs within 48 hours,decrease the chemotaxis of PMNs and THP-1 to PMNs,increase the chemotaxis of FLSs to PMNs.
出处
《华南国防医学杂志》
CAS
2015年第1期4-7,共4页
Military Medical Journal of South China
基金
广东省重大科技专项基金(2012A080201012)
