摘要
为了评价猪白细胞介素-2重组乳酸菌(porcine interleukin-2 recombinant lactic acid bacteria, PIL-2-RLAB)对小鼠小肠结构的影响,试验用磷酸缓冲液(PBS)、乳酸菌(LAB)、PIL-2-RLAB分别灌胃21只4周龄小鼠1周,记录各组小鼠体重增长变化并绘制增重曲线,颈椎脱臼法处理小鼠并取十二指肠进行H.E.染色制作组织切片,显微镜下观察肠绒毛形态变化,测量肠绒毛长度(深度),观察肠上皮细胞形态结构。结果表明:LAB和PIL-2-RLAB均可加快小鼠体重增长,显著促进肠绒毛生长(P<0.05),改善肠上皮细胞结构的完整性。说明PIL-2-RLAB有改善肠组织内部结构,增强机体免疫力的作用,或许可用于动物肠道疾病的防治。
In this study,phosphoric acid buffer(PBS),lactic acid bacteria(LAB),and PIL-2-RLAB were used in 21 4-week old mice for 1 week respectively to record the change of weight growth and draw the weight gain curve of each group of mice.The experiment was conducted to treat the mice by cervical delocation and to take the duodenum H.E.staining for tissue section.The length(depth)of intestinal villi was measured under the microscope and the morphological structure of intestinal epithelial cells was observed.The results show that both of LAB and PIL-2-RLAB can increase the body weight of mice,significantly promote the length(depth)of intestinal villus(P<0.05)and improve the structural integrity of intestinal epithelial cell.It indicates that PIL-2-RLAB can improve intestinal structure,strengthen the body immunity and can be used to prevent and treat the animal intestinal diseases.
作者
刘仲藜
王琳雅
覃嘉兴
赵金
平迪
沙万里
LIU Zhongli;WANG Linya;QIN Jiaxing;ZHAO Jin;PING Di;SHA Wanli(Jilin Agricultural Science and Technology University,Jilin 132101,China;Science and Technology Innovation Center for Ecological Breeding and Disease Prevention and Control for Pigs in Jilin Province,Jilin 132101,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2019年第9期31-33,180,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
吉林农业科技学院大学生科技创新项目(2014010
2017047)
吉林省教育厅项目(2016206)
关键词
猪
白细胞介素-2
重组乳酸菌
免疫增强
组织学观察
porcine
interleukin-2
recombinant lactic acid bacteria
immunological enhancement
histological observation
