摘要
为了研究绿猴肾（Vero）细胞中禽呼肠孤病毒（ARV）的增殖特性,试验采用了光学显微镜观察、半数组织感染量（TCID50）测定、RT-PCR检测等方法对细胞培养物进行了研究。结果表明:ARV S1133毒株在Vero细胞中能有效增殖,并出现细胞破碎、萎缩、抱团、脱落、边缘模糊、胞体变大,甚至死亡等典型的细胞病变（CPE）;RT-PCR检测成功扩增出一条大小为1 248 bp的条带;ARV在感染Vero细胞后会经历三个时期,即潜伏期、快速增长期、稳定期,并在稳定期病毒效价达到峰值,TCID50为1×10-8.46/0.1 m L。
The aim of the present study was to identify the proliferative characteristics of avian reovirus in green monkey kidney cells（ Vero）.The cell culture was analyzed by optical microscopy,semi-tissue infection（ TCID50） and RT-PCR. The results showed that ARV S1133 could effective proliferation in the Vero cells. Several typical cytopathic（ CPE） such as cell disruption,atrophy,aggregation,shedding,edge blur,bigger and even death could be observed on the course of ARV infection in the Vero cells. The gene（ 1 248 bp） was successfully amplified from the infected cells by RT-PCR. After infection with Vero cells,the ARV will undergo three periods: latent period,rapid growth period and stabilization period. The virus titer could reach peak( TCID50= 1×10-8.46/0.1 mL) at the stabilization period.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2018年第1期141-143,254,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(31660715)
国家“万人计划”领军人才专项(2016-37)
中国东盟及一带一路南亚国家动物疫病防控技术研发联合实验室建设项目(桂科AB16380003)
广西自然科学基金项目(2014GXNSFCA118006)
广西水产畜牧兽医局科技项目(桂渔牧科201452003)
广西兽医生物技术室开放基金项目(14-045-31-A-2)
