摘要
城市污泥中含有大量的耐药菌,具有向环境传播耐药菌与抗性基因的潜在危险,但目前缺乏对城市污泥厌氧消化过程中耐药菌的研究.为此对某城市污水处理厂卵形消化池污泥进行了为期1 a的调查研究,考察了中温厌氧消化过程中四环素类与β-内酰胺类抗生素厌氧耐药菌的污染特征与去除效果,并分析了耐药菌的季节性变化规律.结果表明,厌氧进泥中四环素类耐药菌浓度与耐药率均低于β-内酰胺类.中温厌氧消化过程可以去除1.48~1.64 log单位的耐药菌(P<0.05),但经过厌氧消化后氨苄西林与头孢噻吩耐药率有显著增长(分别增加了12.0%与14.3%,P<0.05).厌氧进泥中耐药菌分布有明显的季节特征,除金霉素耐药菌外,其它耐药菌的浓度均为寒冷季显著高于温暖季(P<0.05).
Sewage sludge is one of the major sources that releasing antibiotic resistant bacteria ( ARB) and antibiotic resistant genes ( ARG) into the environment since it contains large amount of ARB, but there is little information about the fate of the anaerobic ARB in the anaerobic digestion of sewage sludge. Therefore, the distribution, removal and seasonal changes of tetracycline and β-lactam antibiotics resistant bacteria in the mesophilic egg-shaped digesters of a municipal wastewater treatment plant were investigated for one year in this study. Results showed that there were higher amounts of ARB and higher resistance rate ofβ-lactam antibiotics than that of tetracycline antibiotics in the sewage sludge. All ARB could be significantly reduced during the mesophilic anaerobic digestion process by 1. 48-1. 64 log unit (P﹤0. 05). Notably, the ampicillin and cephalothin resistance rates were significantly increased after anaerobic digestion by 12. 0% and 14. 3%, respectively ( P ﹤0. 05 ) . The distribution of ARB in the sewage sludge had seasonal change characteristics. Except for chlorotetracycline resistant bacteria, there were more ARB in the sewage sludge in cold season than in warm season (P﹤0. 05).
出处
《环境科学》
EI
CAS
CSCD
北大核心
2014年第10期3814-3820,共7页
Environmental Science
基金
国家高技术研究发展计划(863)项目(2009AA063901)
国家自然科学基金项目(21207147)
中国博士后科学基金项目(2012M510575)
国家水体污染控制与治理科技重大专项(2012ZX07202-005)
江西省科学院省院协同创新团队建设项目(2014SYXTCX02)
关键词
耐药菌
城市污泥
中温厌氧消化
四环素类抗生素
Β-内酰胺类抗生素
antibiotic resistant bacteria(ARB)
sewage sludge
mesophilic anaerobic digestion
tetracyclines
β-lactam antibiotics
