摘要
为了揭示ABR-MBR组合工艺中反硝化除磷微生物种群演替规律,采用Miseq高通量测序技术考察了该工艺在不同运行阶段除磷功能区的微生物群落结构。结果表明,硝化液回流比逐步从150%提升至300%可促进反硝化除磷菌大量富集,促进系统的启动和稳定运行;系统在运行过程中始终保持较高的微生物多样性;优势微生物种群均以变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes)为主,最大丰度分别为55.13%和7.76%,且变形菌门功能性微生物主要集中在γ-变形菌纲(Gamaproteobacteria);功能性除磷菌属主要为气单胞菌属(Aeromonas),假单胞菌属(Pseudomonas)和黄杆菌属(Flavobacterium);其中在逐步提升硝化液回流比过程中气单胞菌属(Aeromonas)被大量富集,其在γ-变形菌纲(Gamaproteobacteria)的相对丰度由5.30%上升至41.49%并在系统后续运行中维持主导地位。系统除磷效果与功能性除磷微生物相对丰度的变化密切相关。系统中微生物种群的多样性和功能微生物的结构稳定性为ABR-MBR工艺的稳定运行和高效处理提供了保证。
In order to reveal the microbial population evolution of denitrifying phosphorus removal in a labscale ABR-MBR combined process,the Miseq high-throughput sequencing technology was used to identify the microbial community structure in phosphorus removal functional zone at different operation stages of the process.Results indicated that the gradual increase of the nitrifying solution reflux ratio from 150%to 300%could promote a large enrichment of denitrifying phosphorus bacteria(DPBs),as well as the startup and stable operation of the system.The system maintained high microbial diversity throughout the operation.The dominant phyla were Proteobacteria and Bacteroidetes,and their maximum abundances were 55.13%and 7.76%,respectively.The main subgroups of the Proteobacteria were related to Gamaproteobacteria.The functional phosphorus removal bacteria were Aeromonas,Pseudomonas and Flavobacterium.Of which Aeromonas was largely enriched during the gradual increase of the nitrifying solution reflux ratio.Its relative abundance in theγ-Proteobacteria phylum(Gamaproteobacteria)increased from 5.30%to 41.49%,and remained dominant in the subsequent operation of the system.The phosphorus removal efficiency of the system was closely related to the change in the relative abundance of functional phosphorus removal microorganisms.The diversity of microbial population and the structural stability of functional microorganisms in the system provided a guarantee for the stable operation and efficient performance of ABR-MBR process.
作者
蒋志云
韦佳敏
缪新年
潘家成
刘文如
沈耀良
JIANG Zhiyun;WEI Jiamin;MIAO Xinnian;PAN Jiacheng;LIU Wenru;SHEN Yaoliang(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment,Suzhou 215009,China;Key Laboratory of Environmental Science and Engineering of Jiangsu Province,Suzhou 215009,China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2019年第7期1653-1661,共9页
Chinese Journal of Environmental Engineering
基金
国家自然科学基金面上资助项目(51578353)
关键词
反硝化除磷
ABR-MBR
微生物群落
高通量测序
硝化液回流比
denitrifying phosphorus removal
ABR-MBR
microbial community characteristics
high-throughput sequencing
nitrite recycling ratio
