摘要
在前期研究中发现,纺锤芽孢杆菌(Bacillus fusiformis,BFN)可以用于降解水溶液的萘,为了解其降解过程,发现BFN菌生长量随着溶液中的萘的含量增加而提高。其中,萘的含量分别是30、50、100和200 mg/L时,BFN的生物量OD600值分别为0.057、0.081、0.126和0.193;降解培养基溶液COD的去除率分别为59.4%、65.3%、69.2%和70.6%,说明BFN菌在生长的过程中利用萘作为碳源。同时,动力学拟合发现,对不同含量萘的降解过程都符合一级降解动力学方程,且BFN菌的生长过程满足逻辑斯蒂方程。扫描电镜图表明,BFN菌在萘的存在下生长得更好。紫外光谱显示波长为276 nm的萘的吸收峰在降解后下降很多。红外光谱数据则表明,降解液中有2组新的吸收峰出现:一组出现在2 878、2 930和2 968 cm-1处,说明在萘的降解过程中有新的羧酸类生成;另一组出现在3 438、3 667和3 731 cm-1处有新的酚类物质生成。
In our previous study,naphthalene was degraded by Bacillus fusiformis(BFN) strain. However,the understanding of degradation of naphthalene is still limited,therefore,in this study,we further explored the process. Results showed that the growth of BFN increased with the increasing concentration of naphthalene. When naphthalene concentrations were 30,50,100 and 200 mg/L,biomass(OD600) were 0. 057,0. 081,0. 126 and 0. 193,respectively. Nevertheless,the removal efficiency of COD decreased with the increasing the concentration of naphthalene,indicating that naphthalene was used as its sole carbon source. The biodegradation of naphthalene fitted well to the first-order kinetic model while the growth kinetics of BFN satisfied well to the logistic model. Furthermore,SEM,UV and FT-IR were employed to characterize the degradation of naphthalene. SEM indicated that the morphology of cells grew better in the presence of naphthalene. UV-vis showed that adsorption peak of naphthalene at 276 nm significantly declined after degradation. Finally,FT-IR data demonstrated that the new bands at 2 878,2 930,2 968,3 438,3 667 and 3 731 cm-1were formed,confirming that carboxyl group and phenolic compounds were existed in degraded solution.
出处
《环境工程学报》
CAS
CSCD
北大核心
2014年第6期2611-2616,共6页
Chinese Journal of Environmental Engineering
基金
福建师范大学闽江学者人才引进基金资助项目(200604)
泉港区科技项目(2013ZH08)
