摘要
目的 探讨正常肝细胞、肝癌细胞、正常肝组织和肝癌组织中连环蛋白 P12 0 (P12 0 ctn)的表达及其在细胞内的分布 ,揭示连环蛋白与肝癌间的关系。方法 采用 RT- PCR、免疫荧光标记 (激光共聚焦显微镜 )和免疫组织化学分析 ,分别对正常肝细胞、肝癌细胞、正常肝组织和肝癌组织中 P12 0 ctn进行检测。结果 RT- PCR检测结果显示 ,上述细胞和组织中均可检测到 P12 0 ctn同工蛋白 1A和 3A m RNA表达 ;免疫荧光标记和免疫组化检测结果显示 ,在正常肝细胞和正常肝组织中 ,P12 0 ctn分布在细胞与细胞连接处和细胞膜上 ,而肝癌细胞和肝癌组织中P12 0 ctn的分布发生了转位 ,胞膜表达减弱或消失 ,胞浆表达增强 ,出现核内转位。结论 P12 0 ctn表达异常与肝癌的发生有一定关系 。
Objective To explore P120 catenin expression in normal liver cell line, hepatocellular carcinoma cell line, normal liver tissue and hepatocellular carcinoma tissues, further exploring the relationship between catenin and hepatocellular carcinoma. Methods Reverse transcript polymerase chain reaction(RT PCR), immunofluorescence microscopy and immunohistochemistry methods were used to detect P120 catenin expression in the cells and tissues mentioned above. Results RT PCR results showed that P120 catenin 1A and 3A isoform mRNA can be detected in all the cells and tissues. Immunofluorescence microscopy and immunohistochemistry results showed that P120 catenin expressed at cell to cell contact region and at cell membrane in normal liver cells and in normal liver tissues, while in hepatocellular carcinoma cell lines and in hepatocellular carcinoma tissues, the molecule expression increased in cytosol, membrane expression reduced, and there was nuclear translocation. Conclusion Hepatocellular carcinoma was found to have relation with abnormal P120 catenin expression in this study, which suggested the process of abnormal cell signaling and adhesion functions during the onset and development of hepatocellular carcinoma.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2003年第1期80-83,共4页
Journal of Sichuan University(Medical Sciences)
基金
广西自然科学基金资助 (桂科自 0 0 0 70 3 7)
