摘要
目的研究山东地区汉族人群的ProfileplusTM9个STR基因座遗传多态性。方法调查山东地区汉族100个无关个体9个STR基因座的基因和基因型频率分布。采用多色荧光标记引物,复合扩增10个基因座,扩增产物进行GeneScan扫描,Genotype分型。建立山东地区汉族9个STR基因座的基因及基因型频率数据库。结果共检测出83种等位基因,其频率分布0.0050～0.4050;220种基因型,其频率分布0.0100～0.2100;平均杂合度(H)为0.7778,累计个体识别能力(DP)为0.9999,非父排除率(EPP)为0.9999,多态信息总量(PIC)为0.5823～0.8396。结论经χ2检验9个STR基因座的基因型分布符合Hardy-Weinberg平衡定律。
Objective To study genetic polymorphism of9STR loci in Han nation populatio n in Shangdong Province.Methods We investigated100unrelated individuals of Han nation population in Shandong Province and studied their genetic distributi on of9STR loci and Amelogenin locus.Primers of10loci were labeled with the N HS -ester fluorescent dye5-FAM(blue),Joe(green)or NED(yellow).The data of Han nation population were generated by multiple amplification and subjected to GeneScan,genotype and genetic distribution analysis.Results83alleles and 220genotypes were observed,with the corresponding frequency of0.0050～ 0.4050and0.0100～0.2100.The average of heterozygosity was0. 7778,the accumulated discrimination power was0.9999.The accumulated probability of exclusion paternity was0.9999.The polymorphism information content was0.5823～0.8396.Conclusions Chi -Square test indicated that the distribution of genotypes agreed with Hardy-Weinberg equilibrium.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2002年第6期620-625,共6页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金(39670399
39970401)
关键词
山东
汉族人群
短串联重复序列
复合扩增
基因扫描
遗传多态性
short tandem repeat
multiple amplification
gene scan
genetic polym orphism
Han nation population in Shangdong Province
