摘要
为了选育大白菜(Brassica campestris L.ssp pekinensis(Lour.)Olsson)抗黑斑病(Alternaria brassicae)新品种和种质创新,在室内利用6个品种的大白菜研究了接种物浓度、苗龄和温度对大白菜黑斑病发病的影响,结果表明,在(25±1)℃光照培养室内,选择浓度为1.0×104个孢子/mL或1.0×105个孢子/mL的黑斑病病菌孢子悬浮液喷雾接种于苗龄为3～4片真叶的大白菜幼苗,保湿24～48 h,10 d后进行病情调查,可对大白菜种质资源抗黑斑病的抗性进行正确评价。
In order to provide technical support for breeding new varieties and germplasm innovation of Chinese cabbage(Brassica campestris L.ssp pekinensis(Lour.) Olsson) with resistance to black spot(Alternaria brassicae),the influence of inoculation spore concentration,seedling stage and temperature on black spot occurrence was studied with six varieties.It was showed that by spraying 1.0×104 pfu/mL or 1.0×105 pfu/mL spore suspension on seedling at 3~4 leaf stage and then moisturizing for 24~48 h at(25±1)℃ in door,the resistance of radish varieties on the black spot could be accurately evaluated 10 d after inoculation.
出处
《湖北农业科学》
北大核心
2012年第23期5363-5365,共3页
Hubei Agricultural Sciences
基金
湖北省农业科技创新中心资助项目(2007-620-007-003)
