摘要
为探讨茼蒿在西瓜枯萎病防治中的作用和效果,并初步探索其作用机制。采用系列稀释法和平板对峙法从水培茼蒿根系悬混液中分离出一株对西瓜枯萎病具有明显拮抗作用的细菌,命名为TK-3。综合菌落形态特征、生理生化特征鉴定和16S r DNA分子鉴定的结果,表明拮抗菌TK-3为解淀粉芽孢杆菌。菌株TK-3中拮抗相关代谢物和生物学性能的研究结果表明:菌株TK-3能够产生吲哚乙酸(IAA)、嗜铁素、NH3和蛋白酶,具备一定的解磷能力,无HCN的产生。盆栽试验结果表明:拮抗菌TK-3对西瓜枯萎病的防治效果达到48.7%。根据以上结果,TK-3菌株是一株极具开发潜力的生防菌株。
To investigate the roles and effects of Chrysanthemum coronarium in disease controlling on watermelon wilt induced by Fusarium oxysporum f. sp. Niveum( FON). An antagonistic bacterium was isolated from root exudate of Chrysanthemum coronarium rhizosphere. This antagonistic bacterium exhibited significantly ability to watermelon wilt pathogen Fusarium oxysporum f. sp. Niveum( FON) based on the plate confrontation experiment. The TK-3 bacterial strains could antagonize Fusarium oxysporum f. sp. Niveum( FON). In a pot-test,Fusarium wilt of watermelon were reduced by TK-3 strain treated in watermelon were 48. 7% of control in 30 days. The results of morphological,physiological and biochemical characterization,and 16 S r DNA-based molecular identification showed that the isolated antagonistic bacterium was Bacillus amylolique-faciens. Production of antibacterial metabolites experiments showed strain TK-3 had the ability of IAA production,siderophore production,NH3 production,proteolytic activity and phosphate solubilization,but could not produce HCN. Results indicated that strain TK-3 had a great potential on bio-control application.
出处
《华北农学报》
CSCD
北大核心
2016年第S1期447-452,共6页
Acta Agriculturae Boreali-Sinica
基金
中央高校基本科研业务费专项资金资助项目(2013PY092)
华中农业大学大学生科技创新基金(SRF)项目
关键词
拮抗细菌
西瓜枯萎病
16S
r
DNA
抑菌活性
解淀粉芽孢杆菌
Antagonistic bacteria
Fusarium wilt of watermelon
16S r DNA identification
Antifungal metabolites
Bacillus amylolique-faciens
