摘要
目的 研究在废用性肌萎缩及其康复过程中大鼠肝细胞雄激素受体 (androgenreceptor ,AR)阳性率的变化 ,探讨AR在肝细胞中的定位及运动对肝组织AR表达的影响。方法 将大鼠随机分为对照组、即刻处死组、自然康复组、跑台训练组及游泳训练组 ,除对照组外 ,其余各组均行石膏固定。固定结束后 ,各组给予相应的康复措施。采用免疫组织化学分析法测定肝细胞中AR的定位及阳性率。结果 AR阳性细胞主要为核着色 ,即刻处死组的AR阳性率显著下降 ,其它各组间差异均无显著性意义。结论 AR主要是一种核受体 ,通过核机制发挥作用 ,运动对肝组织AR表达的影响不大 ,而制动可减少肝组织中AR的含量 ,推测雄激素对肝组织的AR具有正相调节作用。
Objective To investigate the changes of positive ratio of androgen receptor (AR) in hepatic cells during the disuse of skeletal muscle and rehabilitative exercise in rats and to discuss the location of AR in hepatic cells and the effect of exercise on expression of AR in hepatic tissues. MethodsThirty Sprague-Dawley rats were randomized into 5 groups:normal control, immobilization, immobilization plus natural recovery, immobilization plus treadmill exercise, immobilization plus swimming exercise. Immobilization was exerted on the hindlimb of the animals and lasted for 3 weeks. Then 3 weeks of natural recovery or exercise was followed before the sacrifice of the rats for AR test.The location and the positive ratio of AR was determined by immunohistochemical assay. ResultsIt was observed that the positive cells were mostly nucleus stained; and the positive ratio of AR in hepatic cells in rats after immobilization decreased significantly, but there was no significant difference between other groups. ConclusionThe findings of this study suggested AR was largely a kind of nucleus receptor. The effect of exercise on expression of AR in hepatic tissues seemed insignificant and immobilization could reduce the content of AR in hepatic tissues, indicating that androgen had a positive regulation effect on the AR in hepatic tissues.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2002年第12期732-734,共3页
Chinese Journal of Physical Medicine and Rehabilitation
