摘要
目的:探讨电针对脊髓损伤后caspase-3 mRNA及蛋白表达的影响。方法:成年雄性SD大鼠,随机分为模型组、电针(EA)治疗组、甲基强的松龙(MP)组及假手术组。采用改良的Allen's垂击法致大鼠T10脊髓损伤,应用原位杂交和免疫组织化学方法及图象定量分析法观察脊髓损伤后caspase-3 mRNA和蛋白的表达。结果:假手术组caspase-3 mRNA有中等数量的表达,脊髓损伤模型组caspase-3 mRNA表达增高;EA组与MP组caspase-3 mRNA的表达量低于模型组,与其比较有显著性差异;两治疗组间比较无显著性差异。假手术组caspase-3蛋白未见表达,脊髓损伤模型组caspase-3阳性细胞数增多;EA组与MP组caspase-3阳性细胞数低于模型组,与其比较有显著性差异;两治疗组间比较无显著性差异。结论:电针可下调caspase-3 mRNA及蛋白的表达,进而抑制细胞凋亡,保护神经细胞。
Objective: To study the effect of electro-acupuncture on expression of caspase-3 mRNA and protein at early spinal cord injury. Methods:Adult male Sprague-Dawley rats were divided into control group, electro-acupuncture group, Methylprednisolone group and sham opertion group. Moderate injury to T10 spinal cord was abtained by the Allen's method and treated by 2 methods electro-acupuncture and Methylprednisolone. The expression of caspase-3 mRNA and protein were evaluated with in situ hybridization, immunohistochemical analysis and image quantitative analysis. Results: There were moderate caspase-3 mRNA expression,but no protein expression in sham operation group. The expression of caspase-3 mRNA and protein increased in model group. Fewer expression of caspase-3 mRNA and protein in electro-acupuncture groups were noted compared to model rats, and no significant difference was found compared with Methylprednisolone groups. Conclusion:Electric-acupuncture can downregulate the expression of caspase-3 mRNA and protein,it may prevent apoptosis and play a protective role in early spinal cord injury.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2002年第6期548-552,共5页
Chinese Journal of Anatomy
