摘要
目的:探讨caspase-6对成骨肉瘤细胞株的凋亡诱导作用。方法:应用RT-PCR方法检测成骨肉瘤细胞株SOSP-9607中caspase-6基因的表达水平。以腺病毒adv5作载体,构建含caspase-6基因的转基因载体,用脂质体包裹法转染SOSP-9607细胞株,用RT-PCR方法检测转染后SOSP-9607细胞中caspase-6基因的表达。用MTF法检测转染caspase-6对SOSP-9607细胞株生长的影响。结果:成骨肉瘤细胞株SOSP-9607中未检出caspase-6表达。RT-PCR法证实转染。caspase-6后SOSP-9607中caspase-6表达显著增强,MTT检测显示对SOSP-9607细胞的生长有抑制作用,形态学观察及DNA电泳证实转染后的细胞株存在细胞凋亡特征。结论:caspase-6对成骨肉瘤细胞的生长有抑制作用,并可诱导成骨肉瘤细胞凋亡。
Objective:To explore caspase-6's effect on the apoptosis of osteosarcoma cell line SOSP 607. Methods: The expression level of caspase-6 in the osteosarcoma cell line SOSP-9067 was examined by RT-PCR method. The adeno-virus adv5 vector with caspase-6 gene was constructed and transferred into the osteosarcoma cell line SOSP-9607 by lipo-fection. The cell survival rate after transfection was assayed by MTT method. The cell morphological changes were observed by microscope and electron microscope, the apoptosis of transferred cells were examined by gel electrophoresis. Results:No expression of caspase-6 was examined in the osteosarcoma cell line SOSP-9607. A high expression of caspase-6 was identified by RT-PCR after the transfection. The cell growth curve declined after transferring caspase-6. Electrophoresis of DNA displayed the apoptosis ladder. Conclusion: Transferring caspase-6 into the osteosarcoma line SOSP-9607 may inhibit the growth of the osteosarcoma cell line SOSP-9607 and this effect might be achieved by inducing apoptosis.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
2002年第4期261-264,共4页
Chinese Journal of Cancer Biotherapy
基金
第四军医大学博士课题基金
